上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (5): 655-.doi: 10.3969/j.issn.1674-8115.2013.05.029

• 论著（临床研究） • 上一篇    下一篇

ApoA-Ⅰ在多囊卵巢综合征患者卵巢颗粒细胞中的表达

仝腊娟, 丘 彦, 孟江萍, 幸贵邦   

  1. 重庆医科大学附属第一医院辅助生育中心, 重庆 400016
  • 出版日期:2013-05-28 发布日期:2013-05-28
  • 通讯作者: 丘 彦, 电子信箱: cqartq@163.com。
  • 作者简介:仝腊娟（1986—）, 女, 硕士生； 电子信箱: tonglajuan@yahoo.cn。

Expression of ApoA-Ⅰ in granulosa cells in patients with polycystic ovary syndrome

TONG La-juan, QIU Yan, MENG Jiang-ping, XING Gui-bang   

  1. Department of Assistant Reproductive Centre, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
  • Online:2013-05-28 Published:2013-05-28

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摘要：

目的 分析多囊卵巢综合征(PCOS)患者的卵巢颗粒细胞(GCs) 中载脂蛋白A-Ⅰ（ApoA-Ⅰ）的表达情况。方法 收集接受体外授精－胚胎移植治疗的20例PCOS患者（PCOS组）和20例对照组妇女的GCs和术前检查的血清。采用Western blotting技术检测GCs中ApoA-Ⅰ蛋白表达；采用Real-time PCR技术检测GCs中ApoA-ⅠmRNA表达；采用免疫比浊法检测血清ApoA-Ⅰ水平。结果 PCOS组GCs中ApoA-Ⅰ蛋白及mRNA的表达均显著低于对照组(P<0.001，P<0.01)，PCOS组血清ApoA-Ⅰ水平亦显著低于对照组(P<0.05)。结论 PCOS患者GCs中ApoA-Ⅰ表达下调。

关键词: 多囊卵巢综合征, 载脂蛋白A-Ⅰ, 颗粒细胞

Abstract:

Objective To investigate the expression of apolipoprotein A-Ⅰ (ApoA-Ⅰ) in granulosa cells (GCs) of patients with polycystic ovary syndrome (PCOS). Methods Forty women undergoing in vitro fertilization and embryo transfer were divided into PCOS group (n=20) and control group (n=20), and serum samples on preoperative examination day and GCs samples were collected. The expression of ApoA-Ⅰ protein in GCs was detected by Western blotting, the expression of ApoA-Ⅰ mRNA in GCs was determined by Real-time PCR, and the serum level of ApoA-Ⅰ was measured by immunoturbidimetry. Results The expression of ApoA-Ⅰ protein and mRNA in GCs in PCOS group was significantly lower than that in control group (P＜0.001, P＜0.01), and the serum level of ApoA-Ⅰ in PCOS group was also significantly lower than that in control group (P＜0.05). Conclusion The expression of ApoA-Ⅰ is down-regulated in GCs in patients with PCOS.

Key words: polycystic ovary syndrome, apolipoprotein A-Ⅰ, granulosa cells