上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (2): 136-.doi: 10.3969/j.issn.1674-8115.2019.02.006

• 论著·基础研究 • 上一篇    下一篇

枸杞多糖对髓样分化因子 88基因敲除小鼠 2型糖尿病模型炎症因子的影响

王凌霄，刘婷婷，杨晓辉，姚智卿，蔡慧珍   

  1. 上海交通大学基础医学院解剖学与生理学系，上海 200025
  • 出版日期:2019-02-28 发布日期:2019-03-19
  • 通讯作者: 蔡慧珍，电子信箱：xingcao_c@sina.com。
  • 作者简介:王凌霄（1992—），女，硕士生；电子信箱： wanglingxiao92@163.com。
  • 基金资助:
    国家自然科学基金（ 81460494）；宁夏高等学校科学研究项目（ NGY2013072）；2017年宁夏医科大学青年学术骨干人才后备培养项目

Effect of Lycium barbarum polysaccharides on inflammatory cytokines in type 2 diabetes mellitus model mice without myeloid differentiation factor 88 gene

WANG Ling-xiao, LIU Ting-ting, YANG Xiao-hui, YAO Zhi-qing, CAI Hui-zhen   

  1. School of Public Health & Management, NingXia Medical University, Yinchuan 750004, China
  • Online:2019-02-28 Published:2019-03-19
  • Supported by:
    National Natural Science Foundation of China，81460494；Scientific Research Project of Higher Schools in NingXia，NGY2013072；Project for Youth Academic Backbone of NingXia Medical University in 2017

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摘要： 目的 ·研究枸杞多糖（ Lycium barbarum polysaccharide，LBP）对髓样分化因子 88（myeloid differentiation factor 88，MyD88）基因敲除（ MyD88-/-）小鼠 2型糖尿病（ type 2 diabetes mellitus，T2DM）模型炎症因子的影响。方法 ·采用不同浓度 LBP（20、40、 80 mg/kg）干预 MyD88-/-T2DM小鼠， ELISA法检测小鼠血清中白细胞介素 1β（IL-1β）、IL-6、IL-8、转化生长因子 β1（TGF-β1）及 IL-10的水平。采用不同浓度（ 25、50、100 μg/mL）LBP预处理小鼠巨噬细胞系 Raw264.7细胞，然后用脂多糖诱导炎症状态，Western blotting检测各组细胞核因子 κB（NF-κB）的核转位变化，以及 NF-κB抑制蛋白（ IκB）和磷酸化 IκB的蛋白水平。结果 · LBP能够降低 MyD88-/-T2DM小鼠血清 IL-1β和 TGF-β1的水平（均 P<0.05）。体外实验表明， LBP可以剂量依赖性地抑制巨噬细胞中由脂多糖诱发的 NF-κB核转位，同时高剂量的 LBP可以抑制 IκB磷酸化。结论 · LBP可以抑制 MyD88-/-T2DM小鼠部分促炎症因子，这种调节作用可能与其弱化巨噬细胞 IκB磷酸化，抑制 NF-κB核转位有关。

关键词: 枸杞多糖, 髓样分化因子 88, 基因敲除, 2型糖尿病, 巨噬细胞, 炎症因子, 核因子 &, kappa, B

Abstract:

Objective · To investigate the effect of Lycium barbarum polysaccharides on inflammatory cytokines in type 2 diabetes mellitus (T2DM) mice without myeloid differentiation factor 88 gene (MyD88-/-). Methods · Levels of interleukin 1β (IL-1β), IL-6, IL-8, transforming growth factor β1 (TGF-β1), and IL-10 in serum were assessedELISA in the MyD88-/-T2DM mice which had been administered with different doses of LBP (20, 40, and 80 mg/kg). Momacrophages Raw264.7 were stimulatedlipopolysaccharide (LPS) after treatment with different concentrations of LBP (25, 50, and 100 μg/mL). Then Western blotting was used to detect nuclear translocation level of nuclear factor κB (NF-κB) and protein s of inhibitor of NF-κB (IκB) and p-IκB. Results · Serum levels of IL-1β and TGF-β1 in MyD88-/-T2DM mice were down-regulatedLBP (P<0.05). Cell experiment proved that nuclear migration of NF-κB was dose-dependently inhibitedLBP, and the level of p-IκB was reducedhigh dose of LBP. Conclusion · LBP can reduce some proinflammatory cytokines in the MyD88-/-T2DM mice, which may be related with its inhibitive effect on the phosphorylation of IκB and nuclear migration of NF-κB in the macrophages.

Key words: Lycium barbarum polysaccharide (LBP), myeloid differentiation factor 88 (MyD88), gene knockout, type 2 diabetes mellitus (T2DM), macrophage, inflammatory cytokine, nuclear factor &, kappa, B (NF-&, kappa, B)

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