上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

acs-20;acs-22双突变线虫对低渗溶液及小分子药物的敏感性研究

王会子1，韩 冰2，高耀辉3，王立顺2，夏 薇1   

  1. 1.北华大学 医学检验学院， 吉林 132013； 2.上海交通大学 医学院附属瑞金医院分子医学中心， 上海 200025； 3.上海市闵行区中心医院科教科， 上海 201100
  • 出版日期:2013-12-28 发布日期:2014-01-02
  • 通讯作者: 夏 薇, 电子信箱: xiawei4016@126.com。
  • 作者简介:王会子（1988—）， 女， 硕士生; 电子信箱: phoebe.huizi@163.com。

Sensitivity of acs-20;acs-22 double mutant elegans for hypotonic solution and small molecule compounds

WANG Hui-zi1, HAN Bing2, GAO Yao-hui3, WANG Li-shun2, XIA Wei1   

  1. 1.Medical Examination Academy, Beihua University, Jilin 132013, China; 2.Molecular Medicine Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China； 3.Department of Science and Education, Minhang Central Hospital, Shanghai 201100, China
  • Online:2013-12-28 Published:2014-01-02

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摘要：

目的 分析acs-20;acs-22 双突变线虫是否适合经皮肤渗透吸收的小分子化合物的体内效应研究，为建立药物筛选的生物新模型提供线索。方法 野生型秀丽隐杆线虫N2作为对照，PCR验证acs-20;acs-22 双突变线虫2个突变基因（acs-20和acs-22）的缺失。将2种线虫浸泡于低渗溶液（双蒸水）中，记录2种线虫浸后20、40、60 min 时间点的生存率，对比2种线虫对液体渗透压的敏感性。分别用10、20 μg/mL的阿霉素（DOX）处理线虫，记录2种线虫在药物处理后12、24 、48 h时间点的生存率
，对比2种线虫对毒性药物的敏感性。结果 PCR结果显示：与野生型秀丽隐杆线虫N2相比，在acs-20;acs-22双突变线虫DNA序列中确实存在acs-20和acs-22基因缺失，acs-20;acs-22双突变线虫对低渗液体环境和DOX更为敏感。结论 与野生型秀丽隐杆线虫相比，acs-20;acs-22双突变线虫更适合观察经皮肤渗透吸收的小分子化合物的体内效应，可以发展成为一种新的药物筛选生物模型。

关键词: acs-20;acs-22 双突变线虫, 模型, 药物筛选

Abstract:

Objective To investigate acs-20;acs-22 double mutant elegans sensitivity to small molecular compound absorbed through skin penetration, and to provide new model for drug screening. Methods Wild type Caenorhabditis elegans N2 (C. elegans N2) as control, acs-20;acs-22 double mutant elegans was verified by PCR. Both wild type and +acs-20;acs-22 double mutant elegans were soaked in hypotonic solution (double distilled water). The survival rates were recorded at the time of 20, 40, and 60 min to compare the sensitivity of both kinds of elegans to the changes of osmotic pressure. Both elegans were treated with DOX (10 mg/mL and 20 mg/mL) and the survival rates were recorded at the time of 12, 24 and 48 h after treatment. Results PCR results showed that the two relevant genes were successfully mutated in acs-20;acs-22 double mutant elegans compared with the wild type C. elegans N2. And acs-20;acs-22 double mutant elegans was more sensitive to hypotonic solution and DOX treatment. Conclusion Compared to wild type C. elegans N2, acs-20;acs-22 double mutant elegans is more suitable for observing in vivo effect of small molecular compound absorbed through skin penetration, and more appropriate to be used as a new model animal for drug screening.

Key words: acs-20;acs-22 double mutant elegans, model, drug screening