上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

P2Y12受体拮抗剂对裸鼠移植瘤生长的影响

石屹城，宋晓伟，赵仙先，秦永文，荆清   

  1. 第二军医大学附属长海医院心内科， 上海 200433
  • 出版日期:2015-06-28 发布日期:2015-07-30
  • 作者简介:石屹城（1985—）， 男， 硕士生; 电子信箱: 86317251@qq.com。

Effects of P2Y12 receptor antagonist on growth of transplanted tumor of nude mice

SHI Yi-cheng, SONG Xiao-wei, ZHAO Xian-xian, QIN Yong-wen, JING Qing   

  1. Department of Cardiology, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China
  • Online:2015-06-28 Published:2015-07-30

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摘要：

目的 研究P2Y12受体拮抗剂对肿瘤生长的影响。方法 建立HT29结肠癌和MDA231乳腺癌两种裸鼠移植瘤模型，将其随机分为对照组、氯吡格雷组和替格瑞洛组。HT29移植瘤裸鼠对照组及氯吡格雷组各20只，替格瑞洛组19只；MDA231移植瘤裸鼠对照组、氯吡格雷组及替格瑞洛组各4只。分别使用磷酸缓冲液、氯吡格雷混悬液和替格瑞洛混悬液进行灌胃。裸鼠灌胃10 d后处死，测定肿瘤质量和体表面积，观察P2Y12受体拮抗剂对肿瘤生长的影响。结果 HT29移植瘤裸鼠中，对照组肿瘤质量（1.20±0.12）g，体表面积（233.60±20.77）mm2；氯吡格雷组肿瘤质量（0.99±0.08）g，体表面积（192.30±13.28）mm2；替格瑞洛组肿瘤质量（1.04± 0.11）g，体表面积（211.10±19.21）mm2；3组间肿瘤质量及体表面积之间差异无统计学意义（P>0.05）。MDA231移植瘤裸鼠中，对照组肿瘤质量（0.65±0.17）g，体表面积（149.80±27.08）mm2；氯吡格雷组肿瘤质量（1.04±0.24）g，体表面积（183.80±30.18）mm2；替格瑞洛组肿瘤质量（1.08±0.35）g，体表面积（201.30±45.62）mm2；3组间肿瘤质量及体表面积之间差异无统计学意义（P>0.05）。结论 P2Y12受体拮抗剂对裸鼠移植瘤生长无明显促进或抑制作用。

关键词: P2Y12受体拮抗剂, 血小板, 移植瘤模型

Abstract:

Objective To investigate the effects of P2Y12 receptor antagonist on the growth of tumor. Methods Two kinds of nude mice transplanted tumor models HT29 and MDA231 were established. Mice were randomly divided into the control group, clopidogrel group, and ticagrelor group. For HT29 transplanted tumor model, the numbers of nude mice of control group, clopidogrel group, and ticagrelor group were 20, 20, and 19, respectively. For MDA231 transplanted tumor model, the numbers of nude mice of control group, clopidogrel group, and ticagrelor group were all 4. Mice of three groups were intragastric administration with phosphate buffer solution, clopidogrel suspension, and ticagrelor suspension, respectively and sacrificed after 10 d. The weight and body surface area of tumors were measured and the effects of P2Y12 receptor antagonist on the growth of tumor were observed. Results For HT29 transplanted tumor model, the weight and body surface area of tumors of the control group, clopidogrel group, and ticagrelor group were (1.20±0.12) g and (233.60±20.77)mm2, (0.99±0.08) g and (192.30±13.28)mm2, and (1.04±0.11) g and (211.10±19.21) mm2, respectively. The differences of the weight and body surface area of tumors of three groups were not statistically significant (P>0.05). For MDA231 transplanted tumor model, the weight and body surface area of tumors of the control group, clopidogrel group, and ticagrelor group were (0.65±0.17) g and (149.80±27.08) mm2, (1.04±0.24) g and (183.80±30.18) mm2, and (1.08±0.35) g and (201.30±45.62) mm2, respectively. The differences of the weight and body surface area of tumors of three groups were not statistically significant (P>0.05). Conclusion P2Y12 receptor antagonist has no remarkable effect on the growth of transplanted tumor of nude mice.

Key words: P2Y12 receptor antagonist, platelet, transplanted tumor model