上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

miR-506对胃癌细胞增殖和转移的影响

林子龙1,2，周全2，任佳2，李建芳2，俞焙秦2，苏丽萍2，徐岷涓1，刘炳亚1,2   

  1. 1.上海交通大学 系统生物医学研究院， 系统生物医学教育部重点实验室, 上海 200240； 2.上海交通大学 医学院附属瑞金医院， 上海消化外科研究所， 上海市胃肿瘤重点实验室， 上海 200025
  • 出版日期:2016-05-28 发布日期:2016-05-26
  • 通讯作者: 刘炳亚, 电子信箱: liubingya@sjtu.edu.cn。
  • 作者简介:林子龙（1988—）， 男， 硕士生; 电子信箱: linzl0000@gmail.com。
  • 基金资助:

    国家自然科学基金（91529302），国家科技支撑计划（2014BAI09B03）

Effects of miR-506 on the proliferation and metastasis in gastric cancer cells

LIN Zi-long1,2， ZHOU Quan2， REN Jia2， LI Jian-fang2， YU Bei-qin2， SU Li-ping2， XU Min-juan1， LIU Bing-ya1,2   

  1. 1.Shanghai Center for Systems Biomedicine, Ministry of Education Key Laboratory of Systems Biomedicine， Shanghai Jiao Tong University， Shanghai 200240， China； 2.Shanghai Key Laboratory of Gastric Neoplasms， Department of Surgery， Shanghai Institute of Digestive Surgery， Ruijin Hospital， Shanghai Jiao Tong University School of Medicine， Shanghai 200025，China

  • Online:2016-05-28 Published:2016-05-26
  • Supported by:

    National Natural Science foundation of China， 91529302； National Key Technology Research and Development Program of the Ministry of Science and Technology of China， 2014BAI09B03

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摘要：

目的 探讨miR-506对胃癌细胞增殖和转移的抑制作用。方法 采用实时荧光定量PCR 检测5株胃癌细胞系(MKN-45、SGC-7901、BGC-823、NCI-N87和AGS)中miR-506的表达量，以永生化的正常胃黏膜上皮细胞GES-1为对照。通过脂质体转染的方法在SGC-7901细胞系中瞬时转染miR-506和anti-miR-506上调和下调miR-506表达，通过CCK-8实验观察细胞增殖能力改变，通过划痕实验、Transwell迁移和侵袭实验观察细胞运动迁移和侵袭能力的变化。结果 miR-506在各胃癌细胞中的表达量相对于正常胃黏膜上皮细胞GES-1显著降低(P<0.05)。过表达miR-506可显著抑制SGC-7901细胞的增殖、迁移和侵袭能力，与阴性对照的差异有统计学意义(P<0.01)。抑制miR-506表达则可促进增殖、迁移和侵袭能力(P<0.01)。结论 胃癌细胞系中miR-506明显降低，上调miR-506可以抑制胃癌细胞的增殖、迁移和侵袭能力，下调则逆转上述过程。

关键词: miR-506, 胃癌, 增殖, 迁移, 侵袭

Abstract:

Objective To investigate the effects of miR-506 on inhibiting the proliferation and metastasis in gastric cancer cells. Methods The miR-506 expressions in five gastric cancer cell lines (MKN-45, SGC-7901, BGC-823, NCI-N87, and AGS) were measured with real-time fluorescent quantitative PCR. The immortalized human normal gastric mucosa epithelial cell line GES-1 was served as control. MiR-506 mimics and anti-miR-506 were transiently transfected into SGC-7901 cell line to up-regulate and down-regulate the miR-506 expression with liposome transfection method. Changes in the proliferation of SGC-7901 were observed with CCK-8, while cell migration and invasion abilities were observed with wound healing assay and Transwell migration and invasion assay. Results The expressions of miR-506 in five gastric cancer cell lines were significantly lower as compared with normal gastric mucosa epithelial cell line GES-1 (P<0.05). MiR-506 over-expression could significantly inhibit the proliferation, migration, and invasion abilities in SGC-7901 cells. Compared with negative controls, the difference was statistically significant (P<0.01)．The inhibition of miR-506 expression could promote cell proliferation, migration, and invasion abilities (P<0.01). Conclusion The expression of miR-506 significantly decreases in gastric cancer cell lines. Up-regulation of miR-506 can inhibit proliferation, migration, and invasion abilities in gastric cancer cells, while down-regulation of miR-506 has the opposite effects.

Key words: miR-506； gastric cancer； proliferation, migration, invasion