上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (3): 264-.doi: 10.3969/j.issn.1674-8115.2019.03.008

• 论著·基础研究 • 上一篇    下一篇

超声联合 SonoVue微泡对大鼠后肢血管通透性的影响

路灿 1，吉翔 2，耿冠男 3，张素 2，张岚 1，叶猛 1   

  1. 1.上海交通大学医学院附属仁济医院血管外科，上海 200135；2.上海交通大学生物医学工程学院，上海 200030；3.上海交通大学医学院附属仁济医院南院中心实验室，上海 200127
  • 出版日期:2019-03-28 发布日期:2019-04-28
  • 通讯作者: 叶猛，电子信箱：13817145123@163.com。张岚，电子信箱：lucky200207@aliyun.com。为共同通信作者。
  • 作者简介:路灿（1994—），男，硕士生；电子信箱： 2451485087@qq.com。
  • 基金资助:
    上海交通大学医工交叉研究基金（ YG2015MS55）

Effect of ultrasound combined with SonoVue microbubbles on vascular permeability in hind limbs of rats

LU Can1, JI Xiang2, GENG Guan-nan3, ZHANG Su2, ZHANG Lan1, YE Meng1   

  1. 1. Department of Vascular Surgery, Renji Hospital, , Shanghai Jiao Tong University School of Medicine, Shanghai 200135, China; 2. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China; 3. Department of Central Laboratory, The Southern Division of Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2019-03-28 Published:2019-04-28
  • Supported by:
    Shanghai Jiao Tong University Interdisciplinary Research Foundation of Medicine and Engineering， YG2015MS55

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摘要： 目的 ·研究 500 kHz脉冲超声联合 SonoVue微泡对大鼠后肢血管通透性的影响以及通透性改变的时间特点。方法 · 80只 SD雄性大鼠随机分为 8组（每组 10只），分别为空白组、单纯微泡组、微泡 +超声组（包括 2.5 min组、5 min组、10 min组、20 min组、30 min组和 60 min组），其中微泡 +超声组的 6个时间组分别表示超声辐照与伊文思蓝（ Evans blue，EB）注射所间隔的时间。通过检测辐照区肌肉组织中 EB的含量对血管通透性进行定量分析。每组随机选取 4只大鼠，其中 2只大鼠行苏木精 -伊红（hematoxylin-eosin，H-E）染色，另外 2只大鼠行透射电子显微镜检测。结果 ·肌肉内 EB含量检测结果显示，超声辐照联合微泡能够改变大鼠肢体血管的通透性，通透性增加的最佳时间窗为超声辐照后 30 min内，且通透性恢复至正常的时间为 60 min。H-E染色切片未见血管和肌肉组织损伤。透射电子显微镜检测发现微泡 +超声组大鼠局部动脉内皮细胞间的紧密连接显著扩大。结论 · 500 kHz脉冲超声联合 SonoVue微泡能够安全可逆地改变大鼠后肢血管的通透性。

关键词: 超声, 微泡, 血管通透性, 大鼠, 紧密连接

Abstract:

Objective · To investigate the effect of 500 kHz pulsed ultrasound combined with SonoVue microbubbles on the vascular permeability of hind limbs in rats and the permeability dynamics for the duration. Methods · Eighty male Sprague Dawley rats were randomly divided into eight groups (n10), including control group, only microbubble group (MB group), and microbubble combined with ultrasound groups (MB+US group). And MB+US groups classified into time subgroups according to the interval the endpoint of ultrasound exposure to the injection of Evans blue (EB), included 2.5 min group, 5 min group, 10 min group, 20 min group, 30 min group and 60 min group. The change of vascular permeability was quantitively analyzeddetecting the content of EB after ultrasound irradiation. Meanwhile, four rats in each group were randomly selected. Two of them were performed hematoxylin-eosin (H-E) staining; and the rest of them underlied transmission electron microscope analysis. Results · The results of EB content detected muscle samples demonstrated that the exposure of ultrasound in conjunction with microbubbles could alter the vascular permeability of hind limbs of rats. The optimal time window for extravasation was within 30 min after ultrasound exposure. And the permeability could return to the normal status 60 min after ultrasound irradiation. There was no apparent damage on arteries and muscles in the slices with H-E staining. the observation of transmission electron microscope, the tight junctions between endothelial cells on the arterial wall were significantly widened in MB+US groups. Conclusion · The application of ultrasound with the frequency of 500 kHz combined with SonoVue microbubbles can reversibly change the vascular permeability of hind limbs in rats without obvious damage to the tissues.

Key words: ultrasound, microbubble, vascular permeability, rat, tight junction

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