上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2020, Vol. 40 ›› Issue (2): 163-.doi: 10.3969/j.issn.1674-8115.2020.02.004

• 论著·基础研究 • 上一篇    下一篇

白念珠菌ERG3基因敲除及其对耐药性的影响

王钰婷1, 2，刘锦燕2，史 册1，赵珺涛1, 2，项明洁1, 2   

  1. 1. 上海交通大学医学院附属瑞金医院检验科，上海 200025；2. 上海交通大学医学院附属瑞金医院卢湾分院放免检验科，上海 200020
  • 出版日期:2020-02-28 发布日期:2020-03-20
  • 作者简介:王钰婷（1995—），女，硕士生；电子信箱：yutingwang95@163.com。
  • 基金资助:
    国家自然科学基金（81871706）；上海市卫生和计划生育委员会课题（201840227，201740069）；上海市黄浦区卫生和计划生育委员会课题（HKM201702）。

Knocking out ERG3 gene of Candida albicans and its effect on drug resistance

WANG Yu-ting, LIU Jin-yan, SHI Ce, ZHAO Jun-tao, XIANG Ming-jie   

  1. 1. Department of Clinical Laboratory, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2. Radioimmunology and Clinical Laboratory, Luwan Branch, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, China
  • Online:2020-02-28 Published:2020-03-20
  • Supported by:
    National Natural Science Foundation of China (81871706); Shanghai Municipal Planning Commission of Science and Research Fund (201840227, 201740069); Shanghai Huangpu District Municipal Planning Commission of Science and Research Fund (HKM201702).

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摘要： 目的·构建白念珠菌ERG3基因敲除株，分析ERG3敲除对白念珠菌唑类药物耐药性的影响及其常见耐药基因的表达。方法·使用融合PCR和同源重组技术，以白念珠菌SN152 菌株基因组 DNA、带有筛选标记的质粒 DNA 为模板构建敲除组件。采用醋酸锂转染法将敲除组件转染入SN152中，从而获得ERG3+/-和ERG3-/-菌株。采用微量肉汤稀释法来判断SN152、ERG3+/-和ERG3-/-菌株对唑类药物的耐药性差异，同时使用实时荧光定量PCR检测菌株中的常见耐药基因。结果·获得白念珠菌ERG3敲除株。ERG3-/-菌株对唑类药物的耐药性明显高于ERG3+/-和SN152菌株。ERG3-/-菌株的常见耐药基因（CDR1、CDR2、MDR1、ERG11）表达显著上升（均P结论·成功构建白念珠菌ERG3敲除株。ERG3基因的敲除提高了白念珠菌唑类药物的耐药性，且该基因表达下调的同时伴随其他耐药基因的表达上调。

关键词: 白念珠菌, ERG3基因, 基因敲除, 耐药性

Abstract:

Objective · To construct the ERG3 gene knockout strain of Candida albicans, and to analyze the effect of ERG3 knockout on the resistance of Candida albicans to azole drugs and the of its common resistance genes. Methods · Knockout components were constructed with genomic DNA of Candida albicans SN152 and plasmid DNA with screening markers as templatesfusion PCR and homologous recombination techniques. The knockout components were transfected into Candida albicans SN152lithium acetate transfection method to obtain ERG3+/- and ERG3-/- strains. The differences of drug resistance in SN152, ERG3+/- and ERG3-/- strains to azole drugs were determinedmicrobroth dilution method. Meanwhile, real-time quantitative PCR (qPCR) was used to detect the of drug resistance-related genes in the three strains. Results · Candida albicans ERG3 gene knockout strains were efficiently constructed. The resistance of ERG3-/- strains to azole drugs was significantly higher than that of ERG3+/- and SN152 strains. The of drug resistance-related genes (CDR1, CDR2, MDR1, ERG11) in ERG3-/- strains increased significantly (all PConclusion · Candida albicans SN152 ERG3-/- double allelic knockout strain is successfully constructed. The knockout of ERG3 gene improves the resistance of Candida albicans to azole drugs, and the down-regulation of ERG3 gene is accompaniedthe up-regulation of other resistance related genes.

Key words: Candida albicans, ERG3 gene, gene knockout, drug resistance