上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (1): 55-.

• 论著（基础研究） • 上一篇    下一篇

国产13C-美沙西丁呼气试验对小鼠急性肝损伤的评价

陈素芸1, 姜 磊1, 管 樑1, 卢伟京2, 卢 浩2, 杨维成2, 李 彪1, 李培勇1, 杜晓宁2, 李良君2, 朱承谟1   

  1. 1. 上海交通大学医学院 瑞金医院核医学科, 上海 200025；2. 上海化工研究院, 上海 200062
  • 出版日期:2010-01-26 发布日期:2010-01-26
  • 通讯作者: 管 樑, 电子信箱: lanegc2222@yahoo.com。
  • 作者简介:陈素芸（1982—）, 女, 博士；电子信箱: happysue130@gmail.com。
  • 基金资助:

    国家高技术研究发展计划（“八六三”计划）（2008AA02Z427）；上海市科委基金（08dz2200201，08dz2200203）

Evaluation of mouse acute liver damage by breath test with domestically synthesized 13C-methacetin

CHEN Su-yun1, JIANG Lei1, GUAN Liang1, LU Wei-jing2, LU Hao2, YANG Wei-cheng2, LI Biao1, LI Pei-yong1, DU Xiao-ning2, LI Liang-jun2, ZHU Cheng-mo1   

  1. 1. Department of Nuclear Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China；2. Shanghai Research Institute of Chemical Industry, Shanghai 200062, China
  • Online:2010-01-26 Published:2010-01-26
  • Supported by:

    National Hi-Tech Research and Development Program of China, “863” Program, 2008AA02Z427；Shanghai Science and Technology Committee Foundation, 08dz2200201, 08dz2200203

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摘要：

目的 建立一套简单易操作并在小鼠活体上进行的13C呼气试验检测系统，并探讨国产13C-美沙西丁呼气试验（13C-MBT）对小鼠急性肝损伤的诊断价值。方法 13C-美沙西丁经扑热息痛甲烷化合成标记。采用四氯化碳（CCl4）腹腔注射制作小鼠急性肝损伤模型，造模后饲养1个月复制小鼠急性肝损伤恢复模型，行肝脏病理组织学检查和肝功能血液生化学指标检测，于多个时间点采集活体小鼠13C-美沙西丁灌胃后呼出的气体，以红外线能谱仪（IRIS）检测并绘制呼气峰值（DOB）曲线。结果 正常对照组小鼠于灌胃给药后6~8 min时呼气13C达到峰值（51.9±2.04），随后缓慢下降至本底值；模型组于灌胃给药后16 min左右达到峰值（26.37±5.74），随后亦缓慢下降至本底值，两组间差异有统计学意义（P<0.05）。当急性肝损伤恢复模型组和对照组小鼠在相同条件下饲养1个月后，重复13C-美沙西丁呼气检测后两组DOB峰值及达峰时间差异无统计学意义（P>0.05）。结论 13C-MBT可在小鼠活体状态下准确、方便地采集和检测呼出气体的13CO2变化，并正确评估其肝功能的损伤和恢复情况。

关键词: 呼气试验, 小鼠, 国产13C-美沙西丁

Abstract:

Objective To establish a convenient 13C-breath test system in live mice, and investigate the value of 13C-methacetin breath test (13C-MBT) in the diagnosis of acute liver damage of mice with domestically synthesized 13C-methacetin. Methods Domestically synthesized 13C-methacetin was prepared from acamol by methylation. Abdominal injection of CCl4 was adopted to duplicate acute liver damage of mice, then the mice were housed under normal laboratory condition for a whole month to gain recovery, which were indentified by hepatic pathological examinations and biochemical tests of liver function. After fasting, the mice were orally administered 13C-methacetin, and the expired air was collected at various time points. Infrared spectrometer was employed, and delta over baseline (DOB) curves of 13C-exhalation were drawn. Results Six to eight min after administration of 13C-methacetin, the rate of 13C-exhalation peaked in control group (51.9±2.04), and decreased thereafter. Sixteen min after administration of 13C-methacetin, the rate of 13C-exhalation peaked in model group (26.37±5.74), and decreased thereafter. There were significant differences between these two groups (P<0.05). There was no significant difference in peak value and time to reach the peak on DOB curves of 13C-methacetin breath test after the two groups of mice were housed under the same condition for a month (P>0.05). Conclusion 13C-MBT facilitates the collection and evaluation of 13CO2 in the expired air of live mice, and yields precise reflection of alterations of liver function in acute liver injury and functional recovery.

Key words: breath test, mice, domestically synthesized 13C-methacetin