上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (5): 570-.

• 论著（临床研究） • 上一篇    下一篇

非小细胞肺癌PHDs mRNA表达及与Bax和Bcl-2的关系

陈苏峰1, 张 杰2, 朱晓丽3, 姚仕华1, 张军华1, 陈海泉2   

  1. 1.上海交通大学 第六人民医院心胸外科, 上海 200233；2.复旦大学 肿瘤医院胸外科, 上海 200032；3.复旦大学 肿瘤医院病理科, 上海 200032
  • 出版日期:2010-05-25 发布日期:2010-05-28
  • 作者简介:陈苏峰（1970—）, 男, 主治医师, 博士生；电子信箱: chen_sufeng@msn.com。

Relationship between expression of PHDs mRNA and Bax and Bcl-2 in non-small cell lung cancer

CHEN Su-feng1, ZHANG Jie2, ZHU Xiao-li3, YAO Shi-hua1, ZHANG Jun-hua1, CHEN Hai-quan2   

  1. 1.Department of Cardiothoracic Surgery, The Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China；2.Department of Thoracic Surgery, 3.Department of Pathology, Shanghai Cancer Center, Fudan University, Shanghai 200032, China
  • Online:2010-05-25 Published:2010-05-28

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摘要：

目的 研究非小细胞肺癌中脯氨酰羟化酶基因（PHDs) mRNA的表达及其与凋亡相关蛋白Bax和Bcl-2表达的关系。方法 分别采用Real-time PCR技术和免疫组织化学法,检测56例非小细胞肺癌患者肿瘤组织和癌旁正常组织PHD1、2、3基因的表达及癌组织Bax、Bcl-2的表达。结果 56例非小细胞肺癌患者中，肺癌组织PHDs mRNA表达均高于癌旁正常组织，差异均有统计学意义（P<0.05）。Bax和Bcl-2阳性表达病例分别占33.9%（19/56）和32.1%（18/56）。PHD3 mRNA高表达和低表达癌组织中Bcl-2阳性表达率分别为19.5%和66.7%，差异有统计学意义(P<0.05)。结论 非小细胞肺癌组织中PHDs mRNA表达增强。PHD3 mRNA表达上调可能与细胞凋亡有关。

关键词: 脯氨酰羟化酶, 非小细胞肺癌, 凋亡, Bcl-2, Bax

Abstract:

Objective To investigate the expression of prolyl hydroxylase domain enzymes genes (PHDs mRNA), and explore its relationship with expression of apoptosis-related protein Bax and Bcl-2 in non-small cell lung cancer. Methods The expression of PHD1, 2, 3 mRNA and Bax and Bcl-2 in cancer tissues and para-cancerous normal tissues in 56 cases of non-small cell lung cancer was detected by Real-time PCR and immunohistochemical staining, respectively. Results The expression of PHDs mRNA in cancer tissues was significantly higher than that in para-cancerous normal tissues (P<0.05). Patients with positive expression of Bax and Bcl-2 accounted for 33.9% (19/56) and 32.1% (18/56), respectively. The positive expression rates of Bcl-2 in cancer tissues with high expression and low expression of PHD3 mRNA were 19.5% and 66.7%, respectively. Conclusion The expression of PHDs mRNA is increased in nonsmall cell lung cancer tissues, and the increased expression of PHD3 mRNA may be associated with cell apoptosis.

Key words: prolyl hydroxylases, non-small cell lung cancer, apoptosis, Bcl-2, Bax