›› 2010, Vol. 30 ›› Issue (11): 1338-.doi: 10.3969/j.issn.1674-8115.2010.11.005

• 论著(基础研究) • 上一篇    下一篇

冬眠合剂对严重烫伤大鼠早期肺组织的保护作用

邵庆波, 章 雄, 陈雪莲, 刘 琰, 张 勤, 廖镇江   

  1. 上海交通大学 医学院瑞金医院灼伤整形科, 上海 200025
  • 出版日期:2010-11-25 发布日期:2010-11-29
  • 通讯作者: 章 雄, 电子信箱: xiong@medmail.com.cn。
  • 作者简介:邵庆波(1983—), 男, 硕士生;电子信箱: kingboshao@yahoo.cn。
  • 基金资助:

    上海交通大学自然科学基金(2007XJ012)

Protective effect of lytic cocktail on lung tissues of severely scalded rats at initial stage

SHAO Qing-bo, ZHANG Xiong, CHEN Xue-lian, LIU Yan, ZHANG Qin, LIAO Zhen-jiang   

  1. Department of Burns and Plastic Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2010-11-25 Published:2010-11-29
  • Supported by:

    Natural Science Foundation of Shanghai Jiaotong University, 2007XJ012

摘要:

目的 探讨冬眠合剂对严重烫伤大鼠早期肺组织的保护作用及可能机制。方法 66只雄性SD大鼠随机分为假烫伤组(n=6,模拟烫伤+12 mL/kg生理盐水皮下注射)、单纯烫伤组(n=30,Ⅲ度烫伤+12 mL/kg生理盐水皮下注射)和烫伤+冬眠合剂组(n=30,Ⅲ度烫伤+12 mL/kg冬眠合剂皮下注射)。于模拟烫伤后3 h(假烫伤组)和烫伤后3、6、12、24、48 h时点(单纯烫伤组和烫伤+冬眠合剂组)分批采集大鼠血样,处死动物后留取并制备肺组织标本。ELISA法测定血浆致炎因子白介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)、抑炎因子白介素10(IL-10)及内皮细胞表面标志物可溶性细胞间黏附分子1(sICAM-1)和内皮素1(ET-1)水平;酶学分光光度法和免疫组织化学法分别检测肺组织中性粒细胞髓过氧化物酶(MPO)活性和巨噬细胞表面标志物CD68表达。HE染色光学显微镜观察肺组织病理学改变。结果 血清IL-1β、TNF-α、sICAM-1和ET-1水平及肺组织MPO活性和CD68阳性表达率,单纯烫伤组>烫伤+冬眠合剂组>假烫伤组;血清IL-10水平,烫伤+冬眠合剂组>单纯烫伤组>假烫伤组;部分时点检测指标组间比较差异有统计学意义(P<0.05或P<0.01)。与单纯烫伤组烫伤后24 h比较,烫伤+冬眠合剂组相应时点的肺间质水肿程度较轻。结论 冬眠合剂可显著减轻严重烫伤早期大鼠肺间质水肿和炎症反应程度,其机制可能与调节炎症反应,减少内皮细胞的活化与损伤有关。

关键词: 烫伤, 内皮细胞, 肺脏, 冬眠合剂, 炎症反应

Abstract:

Objective To investigate the protective effect of lytic cocktail on lung tissues of severely scalded rats at the initial stage, and explore the potential mechanism. Methods Sixty-six male SD rats were randomly divided into sham scald group (n=6, sham scald with subcutaneous injection of 12 mL/kg saline), scald group (n=30, third degree scald with subcutaneous injection of 12 mL/kg saline) and scald+lytic cocktail group (n=30, third degree scald with subcutaneous injection of 12 mL/kg lytic cocktail). Blood samples were obtained 3 h after scald simulation from sham scald group and 3 h, 6 h, 12 h, 24 h and 48 h after scald from scald group and scald+lytic cocktail group, and lung tissues were harvested after sacrifice from each group. Plasma levels of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), interleukin 10 (IL-10), soluble intercellular adhesion molecule 1 (sICAM-1) and endothelin 1 (ET-1) were determined with ELISA, and the activity of myeloperoxidase (MPO) and the expression of CD68 in lung tissues were detected with enzymatic spectrophotometry and immunohistochemistry, respectively. Pathological changes in lung tissues were observed under light microscope with HE staining. Results The plasma levels of IL-1β, TNF-α, sICAM-1 and ET-1, and the activity of MPO and the expression of CD68 in lung tissues in scald+lytic cocktail group were higher than those in sham scald group and lower than those in scald group. The plasma level of IL-10 in scald group was higher than that in sham scald group and lower than that in scald+lytic cocktail group. There were significant differences in part of the above parameters among groups (P<0.05 or P<0.01). Compared with scald group 24 h after scald, pulmonary interstitial edema in scald+lytic cocktail group was alleviated at the corresponding time point. Conclusion Lytic cocktail can effectively alleviate the pulmonary interstitial edema and inflammation of lung tissues of severely scalded rats at the initial stage, and the potential mechanism may relate to the regulation of inflammation and reduction in activation and injury of endothelial cells.

Key words: burn, endothelial cell, lung, lytic cocktail, inflammatory response