上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (8): 1082-.doi: 10.3969/j.issn.1674-8115.2011.08.007

• 专题报道（眼科视觉科学） • 上一篇    下一篇

倍频532激光激发孟加拉红建立色素兔视网膜静脉阻塞模型

王 帅, 宋蓓雯, 吴 强   

  1. 上海交通大学附属第六人民医院眼科, 上海 200233
  • 出版日期:2011-08-28 发布日期:2011-08-29
  • 作者简介:王 帅（1981—）, 男, 住院医师, 博士；电子信箱: owenhuacha@hotmail.com。
  • 基金资助:

    上海交通大学医工（理）交叉重点课题（YG2009ZD203）

Establishment of pigmented rabbit model of retinal vein occlusion by excitation of Rose Bengal with double-frequency laser (532nm)

WANG Shuai, SONG Bei-wen, WU Qiang   

  1. Department of Ophthalmology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
  • Online:2011-08-28 Published:2011-08-29
  • Supported by:

    Shanghai Jiaotong University Foundation, YG2009ZD203

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摘要：

目的 探讨光化学法诱导色素兔视网膜静脉阻塞模型的方法和特点。方法 将16只健康青紫蓝兔随机分为A、B两组，每组8只。经兔耳缘静脉注入孟加拉红（50 mg/kg）后应用倍频532激光光凝视网膜静脉，A组激光能量150 mW光凝双侧视网膜静脉主干15点后，300 mW再照射15点；B组激光能量150 mW光凝双侧视网膜静脉主干40点后，300 mW再照射40点。分别于术前和光凝后15 min、1 d、3 d、7 d、14 d、21 d、28 d行眼底照相和眼底荧光造影检查，并于光凝后1 d时两组各处死1只动物，其余于光凝后28 d处死，摘除眼球行光学显微镜检查。结果 两组均成功诱导视网膜静脉阻塞模型，光凝后第1天B组产生视网膜动、静脉阻塞。光凝后第7天，A组阻塞静脉全部再通，B组有部分阻塞静脉再通。病理检查结果显示：光凝后第1天，两组视网膜静脉内均见血栓形成；光凝后第28天，B组视盘周围视网膜萎缩，结构不清。结论 利用孟加拉红光化学法制作兔视网膜静脉阻塞模型操作简便，随着局部视网膜静脉接受光凝次数的增加，视网膜动脉亦可出现阻塞现象。

关键词: 光化学法, 视网膜静脉阻塞, 动物模型

Abstract:

Objective To investigate the characteristics of pigmented rabbit retinal vein occlusion induced by photochemical method. Methods Sixteen healthy pigmented rabbits were randomly divided into group A and group B, with 8 rabbits in each group. Laser photocoagulation with double-frequency laser (532 nm) on the trunk retinal vein was performed after the injection of Rose Bengal solution (50 mg/kg) via ear marginal vein. Laser reference parameters of group A were as follows: power 150 mW for 15 shots and then 300 mW for another 15 shots. Laser reference parameters of group B were as follows: power 150 mW for 40 shots and then 300 mW for another 40 shots. Fundus photograph and fundus fluorescein angiography were performed before photocoagulation and 15 min, 1 d, 3 d, 7 d, 14 d and 28 d after photocoagulation. One rabbit was sacrificed in each group 1 d after photocoagulation, and the rest were killed 28 d after photocoagulation for light microscopy. Results Retinal vein occlusion models were successfully established in both groups, and retinal artery occlusion was also induced in group B 1 d after photocoagulation. Seven days after photocoagulation, venous recanalization was detected in all occluded veins in group A and in part of the occluded veins in group B. Histopathological examinations indicated that venous thrombus formed in retinal veins in both groups 1 d after photocoagulation, and there was retinal atrophy around the papillary disc 28 d after photocoagulation. Conclusion It is convenient to establish rabbit model of retinal vein occlusion by Rose Bengal photochemical method. With the increase of photocoagulation shots on regional retinal vein, the retinal artery could be occluded as well.

Key words: photochemistry, retinal vein occlusion, animal model