Journal of Shanghai Jiao Tong University (Medical Science) ›› 2022, Vol. 42 ›› Issue (11): 1534-1541.doi: 10.3969/j.issn.1674-8115.2022.11.003
• Basic research • Previous Articles
YUAN Yongmei(), CHENG Xiaodan, SUN Jiaan, CHANG Dongge, HE Yingying, LIU Chang()
Received:
2022-04-21
Accepted:
2022-08-21
Online:
2022-11-28
Published:
2023-01-04
Contact:
LIU Chang
E-mail:yuanyongmei1977@163.com;lcjzk0@163.com
Supported by:
CLC Number:
YUAN Yongmei, CHENG Xiaodan, SUN Jiaan, CHANG Dongge, HE Yingying, LIU Chang. lncRNA NEAT1 affects the proliferation, invasion and migration of ox-LDL-induced human vascular smooth muscle cells through miR-377-3p/Wnt pathway[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2022, 42(11): 1534-1541.
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URL: https://xuebao.shsmu.edu.cn/EN/10.3969/j.issn.1674-8115.2022.11.003
Gene | Forward sequence (5'→3') | Reverse sequence (5'→3') |
---|---|---|
NEAT1 | CAATGACTTGGGGATGATGCAAACAATTACTG | GTACTCCCCACCTACACACCCAC |
WNT5A | GCGGGACTTTCTCAAGGACA | CGGCTGCCTATTTGCATCAC |
β-actin | AGTGCGACGTGGACATCCG | TGGCTCTAACAGTCCGCCTAG |
miR-377-3p | GGGAATCACACAAAGGCAAC | GTGCGTGTCGTGGAGTCG |
U6 | GCTTCGGCAGCACATATACTAAAAT | CGCTTCAGAATTTGCGTGTCAT |
Tab 1 Primer sequences of each gene
Gene | Forward sequence (5'→3') | Reverse sequence (5'→3') |
---|---|---|
NEAT1 | CAATGACTTGGGGATGATGCAAACAATTACTG | GTACTCCCCACCTACACACCCAC |
WNT5A | GCGGGACTTTCTCAAGGACA | CGGCTGCCTATTTGCATCAC |
β-actin | AGTGCGACGTGGACATCCG | TGGCTCTAACAGTCCGCCTAG |
miR-377-3p | GGGAATCACACAAAGGCAAC | GTGCGTGTCGTGGAGTCG |
U6 | GCTTCGGCAGCACATATACTAAAAT | CGCTTCAGAATTTGCGTGTCAT |
Fig 6 Western blotting detection of cell proliferation and EMT marker proteins expression in each groupNote: A. Control group. B. Model group. C. NEAT1 siRNA group. D. miR-377-3p inhibitor group. E. Transfection negative control group. F. Transfection+inhibition group.
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