上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (12): 1711-.doi: 10.3969/j.issn.1674-8115.2011.12.011

• 论著（基础研究） • 上一篇    下一篇

癫癎持续状态大鼠海马组织中肿瘤坏死因子α和核因子κB的变化及其相关性

宋莎莎, 耿 直, 高 华   

  1. 上海交通大学附属第六人民医院神经内科, 上海 200233
  • 出版日期:2011-12-28 发布日期:2012-01-04
  • 通讯作者: 耿 直, 电子信箱: gengzhi1998@163.com。
  • 作者简介:宋莎莎（1987—）, 女, 硕士生；电子信箱: sss_jiangbian@yahoo.com.cn。

Expression of tumor necrosis factor-alpha and nuclear factor-κB and their correlation in hippocampus of rats with status epilepticus

SONG Sha-sha, GENG Zhi, GAO Hua   

  1. Department of Neurology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
  • Online:2011-12-28 Published:2012-01-04

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摘要：

目的 探讨癫持续状态（SE）大鼠海马组织中肿瘤坏死因子α（TNF-α）和核因子κB（NF-κB）的表达。方法 对Wistar大鼠用10％匹罗卡品300 mg／kg腹腔注射（SE组，n=20），产生SE后60 min终止发作；另设注射等量生理盐水的对照组（n=6）。饲养24 h后取大鼠海马组织，苏木精－伊红染色观察海马CA3区神经元数量，Western blotting测定海马组织NF-κB表达，双抗体夹心酶联免疫法(ELISA)测定海马组织TNF-α表达。采用Pearson相关性分析法分析TNF-α和NF-κB表达的相关性。结果 SE组海马CA3区神经元数量（53.26±2.67）明显小于对照组（103.22±0.83），差异具有统计学意义（P<0.001）。SE组大鼠海马组织TNF-α和NF-κB表达水平均显著高于对照组（P<0.001和P<0.05），且两者表达水平呈正相关（r=0.457，P=0.02）。结论 SE后24 h, TNF-α和NF-κB在大鼠海马组织中表达活性增强，且两者表达水平呈正相关。

关键词: 癫持续状态, 肿瘤坏死因子α, 核因子κB, 大鼠

Abstract:

Objective To investigate the expression of tumor necrosis factor-α (TNF-α) and nuclear factor (NF-κB) in hippocampus of rats with status epilepticus (SE). Methods Twenty Wistar rats were intraperitoneally injected with 10% pilocarpine (300 mg/kg)(SE group),and injection was ceased 60 min after SE onset. Besides, six rats treated with same amount of normal saline were served as control group. Hippocampus tissues of rats were obtained 24 h after treatment, the number of neurons in CA3 area of hippocampus was observed with HE staining, the expression of NF-κB and TNF-α in hippocampus tissues was determined by Western blotting and ELISA respectively. Pearson correlation analysis was adopted to explore the correlation between the expression of TNF-α and that of NF-κB. Results The number of neurons in CA3 area of hippocampus in SE group was much smaller than that in control group (53.26±2.67 vs 103.22±0.83, P<0.001）. The expression of TNF-α and NF-κB in hippocampus tissues in SE group was significantly higher than that in control group （P<0.001, P<0.05）, and there was a positive correlation between the expression of TNF-α and that of NF-κB in SE group （r=0.457，P=0.02）. Conclusion The expression of TNF-α and NF-κB in hippocampus tissues of rats increases 24 h after SE, and there is a positive correlation between the expression of TNF-α and that of NF-κB.

Key words: status epilepticus, tumor necrosis factor-α, nuclear factor-κB, rat