上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

630~650 nm光照射对糖尿病小鼠创面愈合的影响

毛和水，姚 敏，俞为荣，杨鹏高，王 宁，卢化祥，方 勇   

  1. 上海交通大学 医学院附属第三人民医院烧伤整形科 上海交通大学医学院创伤医学研究所， 上海 201900
  • 出版日期:2013-09-28 发布日期:2013-09-29
  • 通讯作者: 方 勇, 电子信箱: fangyong1020@hotmail.com。
  • 作者简介:毛和水（1982—），男，硕士; 电子信箱: mhes1982@163.com。

Effect of 630-650 nm visible light on wound healing in diabetic mice

MAO He-shui, YAO Min, YU Wei-rong, YANG Peng-gao, Wang Ning, LU Hua-xiang, FANG Yong   

  1. Department of Burn and Plastic Surgery, the Third People's Hospital, Institute of Traumatic Medicine, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China
  • Online:2013-09-28 Published:2013-09-29

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摘要：

目的 探讨弱激光疗法（630~650 nm可见光照射）对糖尿病小鼠创面炎症反应及愈合的影响。方法 20只BKS.Cg-m+/+LeprdbNJU小鼠背部以脊柱为对称轴，左右对称各制备直径为8 mm的圆形全层皮肤缺损创面（左侧实验组与右侧对照组，n=20）。实验组予以630~650 nm光照射处理，对照组用普通LED灯照射。分别于创面制备后（伤后）第1、3、7、10、14和21天，观察两组动物创面情况并计算创面愈合率；免疫组织化学染色观察创面中性粒细胞和巨噬细胞浸润情况；ELISA法测定创面组织肿瘤坏死因子α（TNF-α）和白介素6（IL-6）的表达。结果 从伤后第3天开始，实验组创面愈合率均显著高于对照组；与对照组比较，实验组创面中性粒细胞和巨噬细胞的浸润数量在伤后第1、3、7天时明显增多，在伤后第7、14和21天时则显著减少；伤后第3天开始，实验组创面组织IL-6和TNF-α的表达显著低于对照组（P<0.05）。结论 630~650 nm可见光照射能加速糖尿病小鼠创面的炎症反应进程，避免炎症反应的迁延，对创面愈合具有促进作用。

关键词: 弱激光疗法, 630~650 nm可见光, 创面愈合, 炎症反应, 糖尿病

Abstract:

Objective To investigate the effect of 630-650 nm visible light on inflammatory response and wound healing in diabetic mice. Methods Twenty BKS.Cg-m+/+LeprdbNJU mice were used as animal model, and wounds were made on the left and right back of each mice with round fullthickness skin defects of 8 mm in diameter. The experiment group (wounds on the left, n=20) were treated by 630-650 nm visible light, and the control group (wounds on the right, n=20) were treated with normal LED light irradiation. The conditions of wounds and rates of wound healing were observed and recorded 1, 3, 7, 10, 14 and 21 d after injury, the infiltration of neutrophils and macrophages was assessed by immunohistochemical staining, and the expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was determined by ELISA. Results From the third day after injury, the rate of wound healing in experiment group was significantly higher than that in control group. The numbers of infiltrated neutrophils and macrophages in wounds in experiment group were significantly bigger than those in control group 1, 3 and 7 d after injury, and were significantly smaller than those in control group 7, 14 and 21 d after injury. Since the third day after injury, the expression of IL-6 and TNF-α in wounds in experiment group was significantly lower than that in control group (P<0.05). Conclusion For the wounds of diabetic mice, 630-650 nm visible light may accelerate the process of inflammatory response, prevent the persistence of inflammatory response, and promote the wound healing.

Key words: low level laser/light therapy, 630-650 nm visible light, wound healing, inflammatory response, diabetes mellitus