上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (3): 288-.

• 论著（基础研究） • 上一篇    下一篇

结直肠癌组织CypB表达及其对癌细胞迁移和侵袭能力的影响

乐 飞, 郑民华, 陆爱国, 李建文, 王明亮   

  1. 上海交通大学 医学院瑞金医院普外科, 上海 200025
  • 出版日期:2010-03-25 发布日期:2010-03-24
  • 作者简介:乐 飞（1981—）, 男, 博士生；电子信箱: yue.fei@163.com。
  • 基金资助:

    国家自然科学基金（30873000）；上海交大医学院博士创新基金（BXJ0807）

Expression of CypB in colorectal cancer tissues and its effects on |metastasis and invasion of cancer cells

YUE Fei, ZHENG Min-hua, LU Ai-guo, LI Jian-wen, WANG Ming-liang   

  1. Department of General Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-03-25 Published:2010-03-24
  • Supported by:

    National Natural Science Foundation of China, 30873000；Ph.D. Grant of Shanghai Jiaotong University School of Medicine, BXJ0807

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摘要：

目的 观察伴或不伴淋巴结转移的结直肠癌组织中亲环素B（CypB）的表达变化，研究CypB质粒敲除对结直肠癌细胞迁移和侵袭能力的影响。方法 采用免疫组织化学法检测结直肠癌组织芯片中59例结直肠癌组织CypB表达，其中31例无淋巴结转移，28例伴淋巴结转移。构建CypB的RNA干扰质粒，测序鉴定后转染结直肠癌细胞系SW1116（SW1116-siCypB），设立阴性对照（SW1116-NC）；Western blotting分析CypB敲除效果。分别采用划痕实验和细胞侵袭实验评估和检测CypB敲除对SW1116细胞迁移和侵袭能力的影响。结果 伴淋巴结转移结直肠癌组织中CypB表达显著高于无淋巴结转移结直肠癌组织（P<0.01）。与SW1116-NC比较，SW1116-siCypB的CypB表达明显减少，细胞迁移和细胞侵袭能力均显著降低，差异均有统计学意义（均P<0.01）。结论 CypB在结直肠癌淋巴结转移过程中具有重要作用，有望成为防治结直肠癌淋巴结转移的分子靶点。

关键词: 亲环素B, 结直肠癌, 淋巴结转移

Abstract:

Objective To investigate the expression of cyclophilin B (CypB) in colorectal cancer tissues with or without lymph node metastasis, and explore the effects of CypB plasmids knockdown on metastasis and invasion of cancer cells. Methods The expression of CypB in 59 colorectal cancer tissue microarray, including 31 without lymph node metastasis and 28 with lymph node metastasis, were evaluated with immunohistochemistry. Colorectal cancer cell line SW1116 was transfected with shRNA plasmids targeting CypB(SW1116-siCypB) and negative control plasmids(SW1116-NC). CypB knockdown effects were analysed with Western blotting. Wound healing assay and transwell cell invasion assay were performed to observe the effects of CypB knockdown on metastasis and invasion of SW1116 cells. Results The expression of CypB in colorectal cancer tissues with lymph node metastasis was significantly higher than that in colorectal cancer tissues without lymph node metastasis (P<0.01). Compared with SW1116-NC, the expression of CypB of SW1116-siCypB significantly reduced (P<0.01), and the performance of metastasis and invasion significantly decreased (P<0.01). Conclusion CypB plays an important role in lymph node metastasis of colorectal cancer, which makes it a potential target for the prevention and treatment of lymph node metastasis of colorectal cancer.

Key words: cyclophilin B, colorectal cancer, lymph node metastasis