上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (6): 624-.

• 专题报道（抑郁障碍研究） • 上一篇    下一篇

MTHFR基因C677T多态性与汉族人群抑郁症的关联性

李则挚1, 禹顺英2, 张 晨1, 苑成梅1, 洪 武1, 王 勇1, 陈 俊1, 黄 佳1, 汪栋祥2, 方贻儒1   

  1. 上海交通大学 医学院附属精神卫生中心 1.心境障碍科, 2.遗传研究室, 上海 200030
  • 出版日期:2010-06-25 发布日期:2010-06-28
  • 作者简介:李则挚（1981—）, 男, 住院医师, 博士生；电子信箱: lizezhi1981@yahoo.cn。
  • 基金资助:

    “十五”国家科技攻关计划（2004BA720A21-02）；国家高技术研究发展计划（“863”计划，2006AA02Z430）；上海市“登山行动计划”（064119533）；上海市公共卫生优秀青年人才培养项目（08GWQ075）

Association of MTHFR gene C677T polymorphisms and depression in Han populations

LI Ze-zhi1, YU Shun-ying2, ZHANG Chen1, YUAN Cheng-mei1, HONG Wu1, WANG Yong1, CHEN Jun1, HUANG Jia1, WANG Dong-xiang2, FANG Yi-ru1   

  1. 1.Division of Mood Disorder, 2.Department of Genetics, Shanghai Mental Health Center, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China
  • Online:2010-06-25 Published:2010-06-28
  • Supported by:

    National Key Technologies R&D Program of “10th Five-Year Plan”, 2004BA720A21-02；Hi-Tech Research and Development Program of China, “863” Program, 2006AA02Z430；Shanghai“Climbing Mountain Action Plan” Program, 064119533；Shanghai Municipal Health Bureau Foundation, 08GWQ075

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摘要：

目的 探讨N5，N10_亚甲基四氢叶酸还原酶（MTHFR）基因C677T多态性与汉族人群抑郁症发生的关系。方法 纳入符合美国精神障碍诊断与统计手册第4版（DSM-IV）诊断标准的抑郁症患者402例和正常对照者600名，均为汉族。入组时对抑郁症患者进行汉密尔顿抑郁量表（HAMD）-17评定，采用PCR-RFLP技术检测MTHFR基因C677T的多态性。结果 抑郁症组与对照组基因型（CC、CT、TT）分布频率差异均有统计学意义（P<0.05）；其中抑郁症组TT基因型频率显著高于正常对照组，差异有统计学意义（P<0.05）。抑郁症组CT和TT基因型患者的HAMD-17评分均显著高于CC型，差异有统计学意义（P<0.05）。结论 MTHFR基因C677T多态性与抑郁症关联，且TT基因型与抑郁症的严重程度相关。

关键词: 抑郁症, N5, N10-亚甲基四氢叶酸还原酶, 基因多态性

Abstract:

Objective To investigate the relationship between N5, N10-methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphisms and depression in Han populations. Methods Four hundred and two patients with major depressive disorder diagnosed with Diagnostic and Statistical Manual of Mental Disorders 4th Edition (DSM-IV) (major depressive disorder group) and six hundred healthy controls (control group) were included. All subjects were Han nationality. Patients in major depressive disorder group were assessed with Hamilton Rating Scale for Depression (HAMD)-17, and MTHFR gene C677T polymorphisms were determined with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Results There were significant differences in genotype (CC, CT, TT) frequencies between major depressive disorder group and control group (P<0.05). The genotype frequency of TT in major depressive disorder group was significantly higher than that in control group (P<0.05). The HAMD-17 scores of patients with CT and TT genotypes were significantly higher than that of patients with CC genotype in major depressive disorder group (P<0.05). Conclusion MTHFR gene C677T polymorphisms are associated with depression, and genotype TT is related to the severity of depression.

Key words: major depressive disorder, N5, N10-methylenetetrahydrofolate reductase, gene polymorphism