上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (3): 279-.doi: 10.3969/j.issn.1674-8115.2011.03.007

• 论著（基础研究） • 上一篇    下一篇

改良三袖套吻合法建立大鼠左肺原位移植模型及其鉴定

朱宏伟, 吴镜湘, 徐美英   

  1. 上海交通大学附属胸科医院麻醉科, 上海 200030
  • 出版日期:2011-03-28 发布日期:2011-03-29
  • 通讯作者: 徐美英, 电子信箱: myxu55@yahoo.com.cn。
  • 作者简介:朱宏伟（1977—）, 男, 主治医师, 硕士生；电子信箱: zhuhongwei06@yahoo.com.cn。
  • 基金资助:

    上海市卫生局青年基金资助项目（2007Y28）

Improvement of rat model of orthotopic left lung transplantation

ZHU Hong-wei, WU Jing-xiang, XU Mei-ying   

  1. Department of Anesthesiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China
  • Online:2011-03-28 Published:2011-03-29
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2007Y28

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摘要：

目的 对三袖套吻合法建立的大鼠肺移植模型进行改进，建立操作简便和稳定有效的大鼠左肺原位移植模型。方法 在预实验的基础上，对三袖套吻合法建立的大鼠肺移植模型进行以下改进：供体采用胸骨正中切口，经肺动脉圆锥处进行低压肺灌注；制作“袖套”时用无损伤血管夹固定套管尾部；套入“袖套”时用无创显微心耳钳钳夹住左肺门，在不离断“病肺”的情况下完成吻合；完善呼吸道管理流程。以手术成功率、手术时间、移植肺功能、肺组织病理学改变等对模型进行评估。结果 完成大鼠左肺原位移植10例，手术成功率80%。供肺灌洗到获取时间为（12±2）min，体外套管时间为（18±3）min，吻合时间为（31±3）min。移植肺再灌注15 min的氧合指数显著高于单肺通气30 min （P<0.05）。移植肺再灌注2 h后的病理学改变符合典型的缺血－再灌注损伤特征。结论 成功改进了大鼠左肺原位移植模型，该模型稳定有效且操作简便。

关键词: 大鼠, 肺移植, 袖套技术

Abstract:

Objective To improve the cuff-like vessel anastomosis technique-based rat model of lung transplantation, and establish a simple and stable rat model of orthotopic left lung transplantation. Methods Based on preliminary experiments, improvement was made from cuff-like vessel anastomosis technique-based rat model of lung transplantation on the following aspects: lungs were extracted from donors through median sternotomy, and were perfused through cut at the cone of the pulmonary artery with low pressure; blood vessel clamps were used to fix the rear part of pipe; micro left auricular clamps were used to clamp lung hilum instead of removing “sick lung” to complete tallies; respiratory tract management was enhanced. The new model was evaluated by successful rate of operation, time of operation, transplanted lung function and pathological changes. Results Ten consecutive rat models of orthotopic left lung transplantation were completed, with the successful rate of 80%. The time of donor lung perfusion-harvest, donor lung vessel anastomosis and recipient vessel anastomosis was (12±2) min, (18±3) min and (31±3) min, respectively. The oxygenation index 15 min after reperfusion was significantly higher than that 30 min after single lung ventilation (P<0.05). The typical pathological changes of ischemia-reperfusion injury were confirmed by pathological examinations 2 h after reperfusion. Conclusion The improved rat model of orthotopic left lung transplantation has been successfully established, which is simple and effective.

Key words: rat, lung transplantation, cuff technique