上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

DHA对P.gingivalis LPS诱导人牙周膜成纤维细胞炎症反应的影响

周子超，孙梦君，束蓉   

  1. 上海交通大学  医学院附属第九人民医院口腔医学院牙周病科， 上海市口腔医学研究所， 上海市口腔医学重点实验室， 上海 200011
  • 出版日期:2016-05-28 发布日期:2016-05-26
  • 作者简介: 周子超（1990—）， 男， 住院医师， 硕士; 电子信箱: 13917972906@163.com。
  • 基金资助:

    国家自然科学基金（81570977）

Effects of DHA on inflammatory response in human periodontal ligament cells induced by P.gingivalis  LPS

ZHOU Zi-chao, SUN Meng-jun, SHU Rong   

  1. Department of Periodontology, Shanghai Ninth Peoples Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Research Institute of Stomatology, Shanghai Key Lab of Stomatology, Shanghai 200011, China
  • Online:2016-05-28 Published:2016-05-26
  • Supported by:

    National Nature Science Foundation of China， 81570977

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摘要：

目的 观察二十二碳六烯酸（DHA）对牙龈卟啉单胞菌（P.gingivalis）脂多糖（LPS）诱导人牙周膜成纤维细胞（hPDLCs）表达炎症因子的影响。方法 体外培养鉴定hPDLCs，采用MTT法观察不同浓度DHA对hPDLCs细胞活性的影响。根据MTT法的结果，选择对细胞活性没有影响的刺激浓度，分别采用real-time PCR和ELISA技术从基因和蛋白水平检测DHA对P.gingivalis LPS诱导hPDLCs表达炎症因子白细胞介素-6（IL-6）、IL-8和IL-1β的影响。结果 在25～100 μmol/L浓度范围内，DHA对hPDLCs细胞活性没有影响，且可呈浓度依赖性下调P.gingivalis  LPS诱导hPDLCs表达的炎症因子。结论 在不影响细胞活性的情况下，DHA可在一定浓度范围内，呈浓度依赖性下调P.gingivalis  LPS诱导hPDLCs表达的炎症因子，表明DHA具有用于牙周炎抗炎治疗的可能性。

关键词: 二十二碳六烯酸, 牙龈卟啉单胞菌脂多糖, 人牙周膜成纤维细胞, 炎症反应

Abstract:

Objective To investigate the effects of docosahexaenoic acid (DHA) on   periodontal ligament cells (hPDLCs)-expressed inflammatory factors induced by Porphyromonas gingivalis (P.gingivalis) lipopolysaccharides (LPS). Methods HPDLCs were cultured and identified in vitro. The effects of different concentrations of DHA on the cell viability were detected with MTT assay. The concentrations with no effect on the cell viability were selected according to the results of MTT assay and the effects of DHA on hPDLCs-expressed inflammatory factors interleukin-6 (IL-6), IL-8, and IL-1β induced by P.gingivalis LPS were detected at gene and protein levels with real-time PCR and ELISA. Results In the range of 25-100 μmol/L, DHA had no effect on the cell viability in hPDLCs and down-regulated hPDLCs-expressed inflammatory factors induced by P.gingivalis LPS in a concentration-dependent manner. Conclusion In a certain range of concentrations, DHA can down-regulated hPDLCs-expressed inflammatory factors induced by P.gingivalis LPS in a concentration-dependent manner without affecting the cell viability, suggesting the possibility of applying DHA to the anti-inflammatory therapy of periodontosis.

Key words: docosahexaenoic acid, Porphyromonas gingivalis, lipopolysaccharides, human periodontal ligament cells, inflammatory response