上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (7): 769-.doi: 10.3969/j.issn.1674-8115.2018.07.009

• 论著·临床研究 • 上一篇    下一篇

老年心肌梗死患者外周血全基因组DNA甲基化和羟甲基化水平与冠状动脉粥样硬化程度的关系

胡春晓，蒋丹，吴世勇，高磊，常广磊，陶红梅，张冬颖   

  1. 重庆医科大学附属第一医院 1.心血管内科，2.实验研究中心，重庆 400016
  • 出版日期:2018-07-28 发布日期:2018-07-30
  • 通讯作者: 张冬颖，电子信箱：zdy.chris@qq.com。
  • 作者简介:胡春晓（1991—），男，硕士生；电子信箱：1027652647@qq.com。
  • 基金资助:
    国家自然科学基金（81570212）；重庆市卫生和计划生育委员会中医药科技项目（ZY201702073）；重庆市研究生科研创新项目（CYB15093）；重庆市渝中区科技计划项目（20140111）；心脏康复与代谢治疗研究基金；中国心血管健康联盟心血管健康研究院“进阶”研究基金项目

Relationship between global DNA methylation and hydroxymethylation levels in peripheral blood of elderly patients with myocardial infarction and the degree of coronary atherosclerosis

HU Chun-xiao, JIANG Dan, WU Shi-yong, GAO Lei, CHANG Guang-lei, TAO Hong-mei, ZHANG Dong-ying   

  1. 1. Department of Cardiovascular Medicine, 2. Department of Laboratory Research Center, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Online:2018-07-28 Published:2018-07-30
  • Supported by:
    National Natural Science Foundation of China, 81570212; Chinese Medicine Science and Technology Project of Chongqing Health and Family Planning Commission, ZY201702073; Postgraduate Research and Innovation Project of Chongqing, CYB15093; Science and Technology Plan Project of Yuzhong District of Chongqing, 20140111; Cardiac Rehabilitation and Metabolic Therapy Research Foundation; “Advanced” Research Foundation Project of Cardiovascular Health Institute, China Cardiovascular Health Alliance

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摘要： 目的·探讨老年心肌梗死（myocardial infarction，MI）患者外周血中全基因组DNA甲基化和羟甲基化的水平与冠状动脉粥样硬化程度的关系。方法·收集43例确诊为MI的老年患者为研究组，同期53例冠状动脉造影检查阴性的老年患者为对照组。采集患者外周血，酶联免疫法测定外周血全基因组DNA甲基化和羟甲基化水平以及血浆中甲基双加氧酶（ten-eleven translocation-2，TET2）浓度，实时荧光定量PCR测定TET2 mRNA表达水平。结果·与对照组相比，MI患者外周血全基因组DNA甲基化和羟甲基化水平显著增加（P0.014，P0.000），血浆TET2浓度及其mRNA表达水平显著增加（P0.004，P0.028）；DNA甲基化和羟甲基化水平与冠状动脉粥样硬化程度显著相关（r0.262，P0.017；r0.360，P0.001）。结论·老年MI患者外周血中全基因组DNA甲基化和羟甲基化的水平增加，其与冠状动脉粥样硬化程度显著相关。

关键词: 老年, 心肌梗死, 甲基化, 羟甲基化

Abstract:

Objective · To investigate the relationship between the levels of global DNA methylation and hydroxymethylation in peripheral blood of elderly patients with myocardial infarction (MI) and the degree of coronary atherosclerosis. Methods · Forty-three elderly patients with MI were enrolled as the study group and 53 elderly patients with negative findings in coronary angiograms during the same period were collected as the control group. Peripheral blood samples were collected. The levels of global DNA methylation and hydroxymethylation in peripheral blood and the concentration of plasma TET2 were detectedELISA. TET2 mRNA was determinedreal-time qPCR. Results · Compared with the control group, the levels of global DNA methylation and hydroxymethylation in peripheral blood of elderly patients with MI were significantly increased (P0.014, P0.000). The concentration of plasma TET2 and its mRNA in patients with MI were significantly higher than those in the control group (P0.004, P0.028).The levels of global DNA methylation and hydroxymethylation in MI group were significantly correlated with the degree of coronary atherosclerosis (r0.262, P0.017; r0.360, P0.001). Conclusion · The levels of global DNA methylation and hydroxymethylation in peripheral blood of elderly patients with MI are significantly increased, which are significantly correlated with the degree of coronary atherosclerosis.

Key words: elderly, myocardial infarction, methylation, hydroxymethylation

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