上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

单羧酸转运蛋白过表达对乳腺癌MDA-MB-231细胞生物学行为的影响

马冬梅，麦 力，吴晓彬，汪长东，余 畅，李海玉，李 韵，黄裕兵，宋方洲   

  1. 重庆医科大学 基础医学院分子肿瘤研究中心， 重庆 400016
  • 出版日期:2013-09-28 发布日期:2013-09-29
  • 通讯作者: 宋方洲, 电子信箱: fzsongcq＠163.com。
  • 作者简介:马冬梅（1989—），女，硕士生; 电子信箱: 649286576@qq.com。
  • 基金资助:

    国家自然科学基金（30800410）

Effect of over-expression of monocarboxylate transporter on biological behavior of breast cancer MDA-MB-231 cells

MA Dong-mei, MAI Li, WU Xiao-bin, WANG Chang-dong, YU Chang, LI Hai-yu, LI Yun, HUANG Yu-bing, SONG Fang-zhou   

  1. Molecular Medicine and Tumor Research Center, Basic Medical College, Chongqing Medical University, Chongqing 400016, China
  • Online:2013-09-28 Published:2013-09-29
  • Supported by:

    National Natural Science Foundation of China，30800410

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摘要：

目的 研究单羧酸转运蛋白（MCT）过表达对乳腺癌MDA-MB-231细胞生物学行为的影响。方法 将真核表达质粒pcDNA3.1/MCT （实验组）及空质粒pcDNA3.1（阴性对照组）分别转染乳腺癌MDA-MB-231细胞，将未处理的细胞作为空白对照组。采用Real-Time PCR和细胞免疫荧光化学法分别检测转染MCT基因的效率；采用MTS法检测转染MCT基因后对乳腺癌MDA-MB-231细胞增殖的影响，同时用吖啶橙染色法检测细胞形态学的改变；采用流式细胞仪检测过表达MCT基因对乳腺癌MDA-MB-231细胞凋亡、坏死和周期的影响；采用Transwell 实验检测转染MCT基因后对乳腺癌细胞侵袭和转移能力的影响。结果 实验组MCT的基因和蛋白水平明显高于的阴性对照组和空白对照组（P<0.01）；MCT基因过表达能抑制乳腺癌细胞MDA-MB-231的增殖，诱导乳腺癌MDA-MB-231细胞的凋亡和坏死，促使细胞周期S期上升和G2期下降，同时能抑制乳腺癌细胞的侵袭和迁移能力（P<0.01或P<0.05）。结论 MCT基因过表达能促进乳腺癌MDA-MB-231细胞的凋亡和坏死，调控细胞周期，同时能抑制细胞的增殖、侵袭和转移能力。

关键词: 单羧酸转运蛋白, MDA-MB-231, 增殖, 凋亡和坏死, 周期, 侵袭和转移

Abstract:

Objective To investigate the effect of over-expression of monocarboxylate transporter (MCT) on biological behavior of breast cancer MDA-MB-231 cells. Methods Breast cancer MDA-MB-231 cells were transfected with eukaryotic expression plasmid pcDNA3.1/MCT (experiment group) and blank plasmid pcDNA3.1 (negative control group), and cells without treatment were served as blank control group. The transfection efficacy of MCT gene was detected by Real-Time PCR and immunofluorescence method. The effect of MCT gene transfection on proliferation of cells was determined by MTS method, and the morphological changes of cells were examined by acridine orange fluorescent staining. The effect of over-expression of MCT on apoptosis, necrosis and cycle of cells was determined by flow cytometry. The effect of MCT gene transfection on invasion and metastasis of cells was examined by Transwell test. Results The expression of MCT gene and protein in experiment group was significantly higher than that in negative control group and blank control group (P<0.01). The over-expression of MCT gene inhibited the proliferation of cells, enhanced the apoptosis and necrosis of cells, decreased cells in G2 cycle and increased cells in S cycle, and inhibited the invasion and metastasis of cells (P<0.01 or P<0.05). Conclusion The over-expression of MCT gene may promote the apoptosis and necrosis of breast cancer MDA-MB-231 cells, regulate cell cycle, and inhibit the proliferation,  invasion and metastasis of cells.

Key words: monocarboxylate transporter, MDA-MB-231, proliferation, apoptosis and necrosis, cycle, invasion and metastasis