上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

苍术麸炒前后对脾虚证大鼠免疫系统及胃肠激素的影响

刘 芬1,2，刘艳菊2，田春漫1   

  1. 1.湖北民族学院中医药学院中药教研室， 恩施 445000； 2.湖北中医药大学 药学院中药化学教研室， 武汉 430065
  • 出版日期:2015-01-28 发布日期:2015-01-29
  • 通讯作者: 刘艳菊, 电子信箱: lyj19659540@sohu.com。
  • 作者简介:刘 芬（1977—）， 女， 博士生; 电子信箱: 1183794354@qq.com。
  • 基金资助:

    国家自然科学基金（81073051）；国家中医药管理局中医药行业科研专项（201007012-2）；中国中医科学院中药研究所自主选题（2011ZDXK-02）

Effects of Rhizoma Atractylodis before and after being fried with bran on immune system and gastrointestinal hormones of rats with experimental spleen deficiency

LIU Fen1,2, LIU Yan-ju2, TIAN Chun-man1   

  1. 1.Department of Chinese Medicine Teaching and Research Section， College of Traditional Chinese Medicine，Hubei Institute for Nationalities, Enshi 445000, China; 2.Department of Chinese Medicine Chemistry Teaching and Research Section， College of Pharmacy， College of Pharmacy of Hubei University of Chinese Medicine， Wuhan 430065, China
  • Online:2015-01-28 Published:2015-01-29
  • Supported by:

    National Natural Science Foundation of China,81073051; Scientific Research Special Project of Traditional Chinese Medicine Industry, State Administration of Traditional Chinese Medicine of China, 201007012-2; Independent Subject Research Project of Institute of Traditional Chinese Medicine, Academy of Traditional Chinese Medicine of China, 2011ZDXK-02

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摘要：

目的 探讨苍术麸炒前后对实验性脾虚证大鼠胃肠激素及免疫功能的影响，明确苍术麸炒法是否有加强苍术干预脾虚证的作用及可能的机制。方法 采用喂饲小承气汤煎剂加饥饱失常的方法建立大鼠脾虚证模型，模型复制成功后动物随机分为正常组、脾虚模型组、生苍术治疗组、麸炒苍术治疗组和多潘立酮组，连续灌胃给药10 d。采用炭末灌胃法行大鼠胃内残留率、小肠推进比的测定，ELISA法测定大鼠血清中胃泌素（GAS）、胃动素（MTL）和血管活性相关肠肽（VIP）的含量，放射免疫法测定大鼠血清中白细胞介素（IL）1、2、6和肿瘤坏死因子α（TNF-α）的含量，并测定大鼠胸腺、脾脏指数及脾淋巴细胞（T、B）增殖率。结果 与正常组比较，模型组大鼠胃肠动力、激素表达及免疫功能均明显异常；与模型组比较，生苍术治疗组、麸炒苍术治疗组和多潘立酮组的胃内残留率明显下降，小肠推进比明显升高，而大鼠血清中GAS、MTL和VIP的含量不同程度升高，同时，血清中IL-1、2、6和TNF-α的含量不同程度升高，而各组大鼠胸腺、脾脏指数及脾淋巴细胞（T、B）增殖率亦不同程度升高，差异均有统计学意义(P<0.05或P<0.01)；与生苍术治疗组比较，麸炒苍术治疗组上述作用更加明显，两组差异均有统计学意义(P<0.05或P<0.01)。结论 苍术提取物可改善脾虚大鼠胃肠动力，调节胃肠激素的分泌及免疫功能，且麸炒苍术作用优于生苍术。

关键词: 苍术提取物, 麸炒, 脾虚证, 胃肠激素, 免疫

Abstract:

Objective To explore the effects of Rhizoma Atractylodis before and after being fried with bran on the gastrointestinal motility and immune function of rats with experimental spleen deficiency syndrome and to confirm if Rhizoma Atractylodis fried with bran can strengthen the effect of Rhizoma Atractylodes on interventing the spleen deficiency syndrome and possible mechanisms. Methods Rats were fed with Xiaochengqi Decoction combined with irregular hunger method to establish the spleen deficiency syndrome model. After the model was successfully copied, rats were randomly divided into the normal group, model group, Rhizome Atractylodes group, Rhizome Atractylodes fried with bran group, and domperidone group. After 10 d of treatment, the residual rate of stomach and small intestinal propulsion ratio of the rats were detected by the charcoal gavage method. The serum gastrin (GAS), motilin (MTL), and vasoactive intestinal peptide (VIP) levels were detected by the ELISA method. The serum interleukin (IL)-1, -2, -6 and tumor necrosis factor (TNF)-α levels were detected by the radioimmunoassaymethod. The indexes of spleen and thymus and the proliferation rate of spleen lymphocytes (T, B) were measured. Results Compared to the normal group, the gastrointestinal dynamics, hormone expressions, and immune function of the model group were significantly abnormal. Compared to the model group, residual rates of stomach of the Rhizome Atractylodes group, Rhizome Atractylodes fried with bran group, and domperidone group significantly decreased; small intestinal propulsion ratios significantly increased; serum GAS, MTL, and VIP levels increased with different degree; serum IL-1, -2, -6 and TNF-α levels also increased with different degree; and indexes of spleen and thymus and proliferation rates of spleen lymphocytes (T, B) increased with different degree and the differences were statistically significant (P<0.05 or P<0.01). Compared to the Rhizome Atractylodes group, the effects of Rhizome Atractylodes fried with bran were more significant and the differences of two groups were statistically significant (P<0.05 or P<0.01). Conclusion Rhizome Atractylodes extract can improve the gastrointestinal motility and regulate gastrointestinal hormone secretion and immune function of experimental spleen deficiency rats. The effects of Rhizome Atractylodes fried with bran are better than those of Atractylodes rhizome.

Key words: Rhizome Atractylodes extract, fried with bran, spleen deficiency syndrome, gastrointestinal hormone, immunity