上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (12): 1348-.doi: 10.3969/j.issn.1674-8115.2019.12.002

• 论著·基础研究 • 上一篇    下一篇

变性Ⅰ型胶原对内皮细胞增殖、迁移及血管形成相关蛋白合成的影响

苏荣家1，王志勇2，王西樵1，刘英开1，董叫云1，宋 菲1，陆树良1   

  1. 1.上海市烧伤研究所，上海200025；2.上海交通大学医学院附属瑞金医院灼伤整形科，上海 200025
  • 出版日期:2019-12-28 发布日期:2020-02-06
  • 通讯作者: 王志勇，电子信箱：wzy10830@rjh.com.cn。
  • 作者简介:苏荣家（1988—），男，住院医师，硕士；电子信箱：sjtusrj@163.com。
  • 基金资助:
    国家自然科学基金（81071567，30872686，81000838）

Effect of denatured collagen type Ⅰ on endothelial cell proliferation, migration and angiogenesis-related proteins

SU Rong-jia, WANG Zhi-yong, WANG Xi-qiao, LIU Ying-kai, DONG Jiao-yun, SONG Fei, LU Shu-liang   

  1. 1. Shanghai Burns Institute, Shanghai 200025, China; 2. Department of Burns and Plastic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2019-12-28 Published:2020-02-06
  • Supported by:
    National Natural Science Foundation of China, 81071567, 30872686, 81000838

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摘要： 目的·探讨变性Ⅰ型胶原对内皮细胞增殖、迁移及血管生成相关蛋白合成的影响。方法·人脐静脉内皮细胞传代培养后分别接种于正常胶原（正常胶原组）、半量正常胶原（半量胶原组）和变性胶原（变性胶原组）包被的培养板上，2 d后用CCK-8法检测细胞增殖活性，划痕实验观察细胞迁移能力，Western blotting检测膜型基质金属蛋白酶-1（membrane-type 1 matrix metalloproteinase，MT1-MMP）和血管内皮细胞生长因子（vascular endothelial growth factor，VEGF）蛋白表达水平，ELISA法检测血管生成素1（angiopoietin 1，Ang-1）和Ang-2的蛋白分泌情况。结果·CCK-8法检测结果显示，变性胶原组细胞的增殖活性显著高于正常胶原组和半量胶原组（均PPPP>0.05）。结论·变性Ⅰ型胶原能促进内皮细胞增殖、迁移，增加MT1-MMP、VEGF、Ang-2的表达，提示变性胶原在创面修复的血管化过程中可能有积极作用。

关键词: 变性胶原, 内皮细胞, 增殖, 迁移, 血管生成

Abstract:

Objective · To reveal the effect of denatured collagen type Ⅰ on the endothelial cell proliferation, migration, and angiogenesis-related proteins . Methods · Human umbilical vein endothelial cells were cultured on the plates coated with normal collagen (normal collagen group), half concentration normal collagen (half collagen group) or denatured collagen (denatured collagen group). CCK-8 assay was performed to test cell proliferation ability two days later. The effect of collagen on cell migration was measuredscratch test. The s of membrane-type 1 matrix metalloproteinase (MT1-MMP) and vascular endothelial growth factor (VEGF) were detectedWestern blotting. The s of angiopoietin 1 (Ang-1) and Ang-2 were measuredELISA. Results · CCK-8 results showed that the proliferation activity of denatured collagen group was significantly higher than that of normal collagen group and half collagen group (PPPConclusion · Denatured collagen type Ⅰ can promote proliferation, migration and MT1-MMP, VEGF, and Ang-2 s in endothelial cells, suggesting that collagen denaturation may play an active role in the process of angiogenesis during wound healing.

Key words: denatured collagen, endothelial cell, proliferation, migration, angiogenesis