上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (10): 1148-.

• 论著（基础研究） • 上一篇    下一篇

自体灭活T细胞免疫后诱导Treg下调的机制

张秋玉1, 吴娟娟2, 林锦骠2, 张壮壮2, 史 媛2, 沈佰华2, 张 艳2, 李宁丽2, 王 利2   

  1. 1. 福建医科大学免疫学系, 福州 350108；2. 上海交通大学 基础医学院上海市免疫学研究所, 上海 200025
  • 出版日期:2009-10-25 发布日期:2009-10-26
  • 作者简介:张秋玉(1974—), 女, 讲师, 博士生；电子信箱: ZhangQY9801@126.com。
  • 基金资助:

    国家自然科学基金（30671945, 30872990）和上海市科委基金（08ZR1412400）

Mechanism of Treg down-regulation by immunization with attenuated activated autologous T cells

ZHANG Qiu-yu1, WU Juan-juan2, LIN Jin-biao2, ZHANG Zhuang-zhuang2, SHI Yuan2, SHEN Bai-hua2, ZHANG Yan2, LI Ning-li2, WANG Li2   

  1. 1. Department of Immunology, Fujian Medical University, Fuzhou 350108, China；2. Shanghai Institute of Immunology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2009-10-25 Published:2009-10-26
  • Supported by:

    National Natural Science Foundation of China, 30671945, 30872990；Shanghai Science and Technology Committee Foundation, 08ZR1412400

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摘要：

目的 探讨自体灭活T细胞免疫注射后诱导体内调节性T细胞（Treg）下调的机制。方法 自体T细胞体外用刀豆蛋白(ConA)刺激活化，照射灭活T细胞给小鼠进行皮下和腹腔免疫（每只小鼠5×106/次），每隔5 d免疫一次，免疫3次后检测小鼠体内Treg的数量及功能；对照组小鼠皮下注射PBS。ELISA法检测血清中抗鼠CD25抗体。免疫小鼠血清分离后经尾静脉注入未免疫小鼠体内，检测受体小鼠Treg的数量及功能变化。结果 免疫小鼠体内CD4+CD25+Foxp3+ Treg数量较对照组减少（P<0.01），抑制功能也显著降低（P<0.01），但血清中抗CD25的抗体增加（P<0.01）。正常小鼠接受免疫小鼠血清后，Treg的数量和抑制功能下调（P<0.01）。结论 采用ConA 活化的自体灭活T细胞免疫，可诱导抗CD25抗体增加，进而减少体内CD4+CD25+Foxp3+ Treg 细胞数量和功能。

关键词: 自体T细胞, 调节性T细胞, CD4, CD25, Foxp3, 抗体, 小鼠

Abstract:

Objective To explore the mechanism of down-regulation of regulatory T cells（Treg） by immunization with attenuated activated autologous T cells. Methods Autologous T cells were activated with ConA in vitro. Mice were immunized subcutaneously and intraperitoneally every 5 days for 3 times (5×106 per time for each mouse), and the number and function of Treg were examined. PBS was subcutaneously injected for control group. Serum level of anti-mouse CD25 antibody was measured by ELISA. The number and function of Treg was detected by serum adoptive transfer and proliferation and inhibition assays. Results Compared with control group, there were less CD4+CD25+Foxp3+ Treg in the mice after immunization (P<0.01), the immunosuppression ability decreased (P<0.01), and the level of anti-CD25 antibody increased (P<0.01). Adoptive transfer of serum from immunized mice to naive mice led to a significant decrease in Treg population and function in recipient mice (P<0.01). Conclusion Immunization with attenuated activated autologous T cells induces more anti-CD25 antibody, which may further down-regulate CD4+CD25+Foxp3+ Treg expansion and function in vivo.

Key words: autologous T cell, regulatory T cell, CD4, CD25, Foxp3, antibody, mouse

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