上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (10): 1384-.doi: 10.3969/j.issn.1674-8115.2011.10.006

• 论著（基础研究） • 上一篇    下一篇

硅酸三钙、β-磷酸三钙和Dycal对牙髓细胞增殖能力的影响

江 龙, 彭伟伟, 朱亚琴   

  1. 上海交通大学 医学院附属第九人民医院·口腔医学院 口腔综合科, 上海市口腔医学重点实验室, 上海 200011
  • 出版日期:2011-10-28 发布日期:2011-10-27
  • 通讯作者: 朱亚琴, 电子信箱: zyq1590@163.com。
  • 作者简介:江 龙（1979—）, 男, 住院医师, 博士生；电子信箱: jianglong25a@163.com。
  • 基金资助:

    上海市科委项目（08DZ2271100，08JC1414500）；上海市教委科研创新重点项目（09ZZ116）；上海高校选拔培养优秀青年教师科研专项基金（jdy09051）

Effects of calcium silicate, β-tricalcium phosphate and Dycal on proliferation of dental pulp cells

JIANG Long, PENG Wei-wei, ZHU Ya-qin   

  1. Department of General Dentistry, the Ninth People's Hospital, School of Stomatology, |Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
  • Online:2011-10-28 Published:2011-10-27
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 08DZ2271100, 08JC1414500；Shanghai Education Committee Foundation, 09ZZ116; Shanghai College Excellent Young Teachers Foundation, jdy09051

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摘要：

目的 比较硅酸三钙（C3S）、β磷酸三钙(β-TCP)和Dycal对牙髓细胞（DPCs）增殖能力的影响。方法 改良组织块法体外传代培养DPCs。制备含不同质量浓度C3S、β-TCP和Dycal的材料浸提液并处理第3代DPCs（C3S、β-TCP、Dycal不同质量浓度组）3 d，MTT比色法检测细胞增殖能力指标光密度值D(490 nm)，以不添加材料浸提液的DPCs作为阴性对照组。分别以6.25 mg/mL的C3S、β-TCP和Dycal 材料浸提液培养第3代DPCs（C3S、β-TCP和Dycal组），以普通培养液培养的DPCs作为对照组，流式细胞仪检测各组培养后第3天和第6天的细胞周期的变化。结果 MTT检测结果显示：培养后3 d，C3S不同质量浓度组的D(490 nm)均显著高于阴性对照组（P<0.05），C3S 0.625 mg/mL组和6.25 mg/mL组的D(490 nm)达峰值；β-TCP不同质量浓度组的D(490 nm)与阴性对照组比较，差异均无统计学意义（P>0.05）；Dycal 50 mg/mL和100 mg/mL组的D(490 nm)均显著低于对照组（P<0.05）。流式细胞仪检测结果显示：培养后第3天和第6天，C3S组S+G2期细胞比例均显著高于对照组、β-TCP组、Dycal组（P<0.05）。结论 C3S具有促进DPCs增殖的作用；β-TCP对DPCs的增殖能力无明显影响，而Dycal对DPCs的增殖具有一定的抑制作用。

关键词: 硅酸三钙, β-磷酸三钙, Dycal, 牙髓细胞, 细胞增殖, 细胞周期

Abstract:

Objective To compare the effects of calcium silicate (C3S), β-tricalcium phosphate (β-TCP) and Dycal on the proliferation of dental pulp cells (DPCs). Methods DPCs were obtained using modified tissue explant technique in vitro. DPCs of the third passage were cultured with material extract fluids containing different mass concentrations of C3S, β-TCP and Dycal for 3 d (different mass concentrations of C3S, β-TCP and Dycal groups), and proliferation-related parameter of optical density [D (490 nm)] was measured by MTT assay. DPCs without culture with material extract fluids were served as negative control group. DPCs of the third passage were cultured with material extract fluids containing 6.25 mg/mL C3S, β-TCP and Dycal respectively (C3S, β-TCP and Dycal groups), those cultured with routine culture fluid were served as control group, and the changes of cell cycles were detected by flow cytometry in each group. Results MTT assay revealed that 3 d after culture, D (490 nm) in different mass concentrations of C3S groups was significantly higher than that in negative control group (P<0.05), D (490 nm) in 0.625 mg/mL group and 6.25 mg/mL group reached the peak, there was no significant difference in D (490 nm) between negative control group and different mass concentrations of C3S groups （P>0.05）, and D (490 nm) in 50 mg/mL Dycal group and 100 mg/mL Dycal group was significantly lower than that in control group (P<0.05). Flow cytometry demonstrated that 3 d and 6 d after culture, the percents of cells in S+G2 stage in C3S group were significantly higher than those in control group, β-TCP group and Dycal group (P<0.05). Conclusion C3S can promote the proliferation of DPCs. β-TCP does not have significant effect on the proliferation of DPCs, while Dycal inhibits the proliferation of DPCs.

Key words: calcium silicate, β-tricalcium phosphate, Dycal, dental pulp cell, proliferation, cell cycle