上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (10): 1398-.doi: 10.3969/j.issn.1674-8115.2011.10.009

• 论著（基础研究） • 上一篇    下一篇

环磷酰胺化疗导致小鼠卵巢功能损伤模型的建立

王方圆1, 谢 超2, 程蔚蔚1, 韩 伟3, 赖东梅1   

  1. 1.上海交通大学 医学院附属国际和平妇幼保健院中心实验室, 上海 200030； 2.上海交通大学 农业与生物学院 上海市兽医生物技术重点实验室, 上海 200240； 3.上海交通大学 药学院再生组学研究室, 上海 200240
  • 出版日期:2011-10-28 发布日期:2011-10-27
  • 通讯作者: 赖东梅, 电子信箱: laidongmei@hotmail.com；韩 伟, 电子信箱: weihan@sjtu.edu.cn。
  • 作者简介:王方圆（1983—）, 女, 硕士；电子信箱: sunflowerwang113@gmail.com。
  • 基金资助:

    上海交通大学医学院附属国际和平妇幼保健院院内基金（3030448）；上海交通大学医工合作面上项目（YG2010MS66）

Establishment of mouse model of cyclophosphamide-induced ovarian failure

WANG Fang-yuan1, XIE Chao2, CHENG Wei-wei1, HAN Wei3, LAI Dong-mei1   

  1. 1.Central Research Laboratory, the International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China；2.Shanghai Municipal Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China；3.Laboratory of Regeneration Medicine, School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China
  • Online:2011-10-28 Published:2011-10-27
  • Supported by:

    Foundation of the International Peace Maternity and Child Health Hospital affiliated to Shanghai Jiaotong University School of Medicine, 3030448；Shanghai Jiaotong University Foundation, YG2010MS66

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摘要：

目的 探索建立化疗后导致小鼠卵巢功能损伤模型的方法。方法 以C57BL/6雌性小鼠为研究对象，以75 mg/kg的剂量一次性腹腔注射环磷酰胺，观察小鼠一般情况、动情周期、血清雌激素（雌二醇）水平以及卵巢组织的形态学改变、各级卵泡数变化、抗苗勒管激素分泌情况和组织纤维化程度。以未注射环磷酰胺小鼠作为对照。结果 与对照组比较，环磷酰胺注射小鼠血清雌二醇水平降低，卵巢组织抗苗勒管激素分泌减少，卵巢组织间质增生、纤维化明显，各级卵泡数目减少。结论 75 mg/kg的剂量一次性腹腔注射环磷酰胺后可引起小鼠明显的卵巢功能损伤，小鼠生殖内分泌和卵巢组织学的变化与临床上化疗导致卵巢功能损伤患者的情况相似。该方法简便易行、造模时间短、成功率高、死亡率低，是建立化疗导致卵巢功能损伤动物模型的较好方法。

关键词: 卵巢功能损伤, 化疗, 动物模型, 小鼠

Abstract:

Objective To establish the mouse model of chemotherapy-induced ovarian failure. Methods Female C57BL/6 mice were intraperitoneally injected with cyclophosphamide at the dose of 75 mg/kg. The general conditions, estrous cycles, serum estrogen (estradiol) levels, morphology of ovarian tissues, changes of numbers of follicles, secretion of anti-Mullerian hormone and tissue fibrosis of mice were observed. Mice without cyclophosphamide injection were served as controls. Results Compared with controls, the serum estrogen level and secretion of anti-Mullerian hormone of ovarian tissues decreased, there were significant stromal hyperplasia and fibrosis in ovarian tissues, and the number of follicles reduced in mice injected with cyclophosphamide. Conclusion Intraperitoneal injection of 75 mg/kg cyclophosphamide may cause obvious ovarian failure, and the changes in reproductive endocrine and ovarian histopathology are similar to those of chemotherapy-induced ovarian failure in female patients in clinics. It can be used to establish the animal model of chemotherapy-induced ovarian failure due to its simplicity, feasibility, high success rate and low mortality.

Key words: ovarian failure, chemotherapy, animal model, mouse