Journal of Shanghai Jiao Tong University (Medical Science) ›› 2023, Vol. 43 ›› Issue (1): 8-19.doi: 10.3969/j.issn.1674-8115.2023.01.002
• Basic research • Previous Articles Next Articles
XIE Lin1,2(), CHENG Ye2(), ZHENG Qimin1, ZHANG Xi2, FU Lili3, CHEN Min2, WANG Yi2, MEI Changlin3, XIE Jingyuan1, GU Xiangchen1,2()
Received:
2022-04-29
Accepted:
2022-12-16
Online:
2023-01-28
Published:
2023-01-28
Contact:
GU Xiangchen
E-mail:18516260652@163.com;hellochengye@163.com;gettygugu@126.com
Supported by:
CLC Number:
XIE Lin, CHENG Ye, ZHENG Qimin, ZHANG Xi, FU Lili, CHEN Min, WANG Yi, MEI Changlin, XIE Jingyuan, GU Xiangchen. Preventive effect of icariin on transition from acute kidney injury to chronic kidney disease in mouse model[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2023, 43(1): 8-19.
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URL: https://xuebao.shsmu.edu.cn/EN/10.3969/j.issn.1674-8115.2023.01.002
Gene | Forward (5′→3′) | Reward (5′→3′) |
---|---|---|
Fn (mouse) | ATGGTACAGCTGATCCTGCC | GCCCTGGTTTGTACCTGCTA |
Acta2 (mouse) | GGAACCCTGAGACGCTGCT | CATTCCAACCATTACTCCC |
Col1a1 (mouse) | CCCAGCCGCAAAGAGTCTAC | AGCATACCTCGGGTTTCCAC |
Tgfb1 (mouse) | GGGAAGCAGTGCCCGAACCC | TGGGGGTCAGCAGCCGGTTA |
Hprt (mouse) | TATGCCGAGGATTTGGAAAA | TCCCATCTCCTTCATGACATC |
Cpt1a (mouse) | CTTCCCATTTGACACCTTTG | ATACGTGAGGCAGAACTTGC |
Col3a1 (mouse) | ACAGCTGGTGAACCTGG | ACCAGGAGATCCATCTCGAC |
Vimentin (mouse) | GGATCAGCTCACCAACGACA | GGTCAAGACGTGCCAGAGAA |
Cpt2 (mouse) | CCCAGGCTGCCTATCCCT | TCCTTCCCAATGCCGTTC |
Ppara (mouse) | AGAGCCCCATCTGTCCTCTC | ACTGGTAGTCTGCAAAACCAAA |
Acox2 (mouse) | GAGGGTGAGAATACGGT | GGTCTTGGTGTGGCGAG |
Ppara (rat) | AACATCGAGTGTCGAATATGTGG | CCGAATAGTTCGCCGAAAGAA |
Tnfa (rat) | ATGGGCTCCCTCTCATCAGT | GCTTGGTGGTTTGCTACGAC |
Fn (rat) | AGAGGGGAGTGGAAGTGTGA | GTTGTAGGTGAACGGGAGAA |
Tgfb1 (rat) | CTAATGGTGGACCGCAACAAC | CACTGCTTCCCGAATGTCTGA |
Ctgf (rat) | AACCGTGTGTCATTGTCAT | CACCTTAGTGTGCGTTCTG |
Cpt1a (rat) | ATGGAGGTTGTCTACGA | CAGGGGTTTACTTTTTA |
Cpt2 (rat) | CAGCTGACCAAAGAAGCAGC | GTTCAGAGTGCTGGTGGACA |
Col1a1 (rat) | CTGACGCATGGCCAAGAAGA | TTGGGTCCCTCGACTCCTAT |
β-actin (rat) | GTAAAGACCTCTATGCCAACA | GGACTCATCGTACTCCTGCT |
Tab 1 Primer sequences for real-time qPCR
Gene | Forward (5′→3′) | Reward (5′→3′) |
---|---|---|
Fn (mouse) | ATGGTACAGCTGATCCTGCC | GCCCTGGTTTGTACCTGCTA |
Acta2 (mouse) | GGAACCCTGAGACGCTGCT | CATTCCAACCATTACTCCC |
Col1a1 (mouse) | CCCAGCCGCAAAGAGTCTAC | AGCATACCTCGGGTTTCCAC |
Tgfb1 (mouse) | GGGAAGCAGTGCCCGAACCC | TGGGGGTCAGCAGCCGGTTA |
Hprt (mouse) | TATGCCGAGGATTTGGAAAA | TCCCATCTCCTTCATGACATC |
Cpt1a (mouse) | CTTCCCATTTGACACCTTTG | ATACGTGAGGCAGAACTTGC |
Col3a1 (mouse) | ACAGCTGGTGAACCTGG | ACCAGGAGATCCATCTCGAC |
Vimentin (mouse) | GGATCAGCTCACCAACGACA | GGTCAAGACGTGCCAGAGAA |
Cpt2 (mouse) | CCCAGGCTGCCTATCCCT | TCCTTCCCAATGCCGTTC |
Ppara (mouse) | AGAGCCCCATCTGTCCTCTC | ACTGGTAGTCTGCAAAACCAAA |
Acox2 (mouse) | GAGGGTGAGAATACGGT | GGTCTTGGTGTGGCGAG |
Ppara (rat) | AACATCGAGTGTCGAATATGTGG | CCGAATAGTTCGCCGAAAGAA |
Tnfa (rat) | ATGGGCTCCCTCTCATCAGT | GCTTGGTGGTTTGCTACGAC |
Fn (rat) | AGAGGGGAGTGGAAGTGTGA | GTTGTAGGTGAACGGGAGAA |
Tgfb1 (rat) | CTAATGGTGGACCGCAACAAC | CACTGCTTCCCGAATGTCTGA |
Ctgf (rat) | AACCGTGTGTCATTGTCAT | CACCTTAGTGTGCGTTCTG |
Cpt1a (rat) | ATGGAGGTTGTCTACGA | CAGGGGTTTACTTTTTA |
Cpt2 (rat) | CAGCTGACCAAAGAAGCAGC | GTTCAGAGTGCTGGTGGACA |
Col1a1 (rat) | CTGACGCATGGCCAAGAAGA | TTGGGTCCCTCGACTCCTAT |
β-actin (rat) | GTAAAGACCTCTATGCCAACA | GGACTCATCGTACTCCTGCT |
Fig 4 Mitochondrial structural damage and expression levels of related mRNAs in the AKI-CKD murine model at remodeling stage and preventive effect of ICA
Fig 9 Effect of ICA on expression levels of fatty acid oxidative metabolism-related mRNAs and the apoptotic protein in renal tubular epithelial cells after knocking down Ppara
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