›› 2018, Vol. 38 ›› Issue (3): 305-.doi: 10.3969/j.issn.1674-8115.2018.03.012

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Pyruvate dehydrogenase activity and pyruvate dehydrogenase kinase 4 expression in skeletal muscle of end-stage renal disease patients

XU Chen-qi1* , MIN Lu-lin1* , CHEN Xiao-huan3 , LU Ren-hua1 , ZHU Ming-li1 , XIE Ke-wei1 , QIAN Ying-ying1 , GUO Xiang-jiang2 , NI Zhao-hui1 , AYIJIAKEN Kasimumali3#, GU Le-yi1#   

  1. 1. Molecular Cell Laboratory for Kidney Diseases, Renal Division, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 2. Department of Vascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 3. Department of Nephrology, Kashgar Second People′s Hospital, Xinjiang Uygur Autonomous Region, Kashgar 844000, China
  • Online:2018-03-28 Published:2018-05-03
  • Supported by:
     Natural Science Foundation of Xinjiang Uygur Autonomous Region，2015211C227

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Abstract: Objective · To explore the changes of pyruvate dehydrogenase (PDH) activity and pyruvate dehydrogenase kinase 4 (PDK4) expression in the end-stage renal disease (ESRD) patients' skeletal muscles. Methods · Skeletal muscle samples were collected from non-chronic kidney disease (nonCKD) patients and ESRD patients. PDH activity was detected by ELISA assay. Real-time qPCR was performed to examine gene transcription levels of PDK1-PDK4 and PDH subunits. Western blotting analysis was used to detect protein expression levels of PDK1 and PDK4. Results · There were no demographic differences between two groups of patients. Plasma creatinine and urea nitrogen were significantly elevated in ESRD group (both P<0.05), while estimated glomerular filtration rate, hemoglobin and plasma albumin in ESRD group were significantly lower than those in non-CKD group (all P<0.05). Skeletal muscle PDH activity in ESRD group was markedly lower than that in non-CKD group (P=0.014). There were no differences in PDK1- PDK4 and PDH subunits mRNA transcription levels between ESRD and non-CKD group. PDK4 protein expression was significantly higher than that in non-CKD group (P=0.000). Conclusion · The decreased PDH activity in ESRD patients' skeletal muscle may be related to up-regulation of PDK4.

Key words: end-stage renal disease (ESRD), skeletal muscle, pyruvate dehydrogenase (PDH), pyruvate dehydrogenase kinase 4 (PDK4), mitochondria