上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (10): 1393-.doi: 10.3969/j.issn.1674-8115.2011.10.008

• 论著（基础研究） • 上一篇    下一篇

雄激素和高脂饮食对雌性大鼠卵巢、垂体、肾上腺超微结构的影响

翟华玲1, 吴 晖1, 张 岚2, 陆颖理1   

  1. 1.上海交通大学 医学院附属第九人民医院内分泌代谢科, 上海200011； 2.上海交通大学 基础医学院生物化学与细胞分子生物学教研室, 上海 200025
  • 出版日期:2011-10-28 发布日期:2011-10-27
  • 通讯作者: 陆颖理, 电子信箱: luy662003@yahoo.com.cn。
  • 作者简介:翟华玲（1979—）, 女, 主治医师, 博士生；电子信箱: zhaihualing@yahoo.com.cn。
  • 基金资助:

    上海交通大学医学院基金（YZ1001）

Effects of androgen and high-fat diet on ultrastructures of ovary, pituitary and adrenal in female rats

ZHAI Hua-ling1, WU Hui1, ZHANG Lan2, LU Ying-li1   

  1. 1.Department of Endocrinology and Metabolism, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai200011, China；2.Department of Biochemical and Cell Molecular Biology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2011-10-28 Published:2011-10-27
  • Supported by:

    Foundation of Shanghai Jiaotong University School of Medicine, YZ1001

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摘要：

目的 研究雄激素和高脂饮食对雌性SD大鼠卵巢、垂体和肾上腺超微结构的影响。方法 24只21日龄雌性SD大鼠随机分为对照组、雄激素注射组和雄激素注射+高脂饮食组。8周后，检测空腹血糖及血清胰岛素、睾酮、三酰甘油和胆固醇水平；透射电子显微镜观察卵巢、垂体、肾上腺组织超微结构。结果 雄激素注射组、雄激素注射+高脂饮食组大鼠血糖、胰岛素、睾酮、三酰甘油、胆固醇水平均高于对照组；卵巢呈多囊性改变，垂体、肾上腺超微结构显示凋亡细胞增多。雄激素注射+高脂饮食组大鼠肾上腺上皮细胞的细胞质内脂滴增多。结论 雄激素可诱导多囊卵巢的形成，同时可导致血糖升高；联合高脂饮食可构建较理想的大鼠多囊卵巢综合征模型。

关键词: 多囊卵巢综合征, 超微结构, 雄激素, 高脂饮食

Abstract:

Objective To investigate the effects of androgen and high-fat diet on ultrastructures of ovary, pituitary and adrenal in female SD rats. Methods Twenty-four female SD rats aged 21 d were randomly divided into control group, androgen injection group and androgen injection+high-fat diet group. Eight weeks after treatment, serum concentrations of glucose, insulin, testosterone, triglyceride and cholesterol were measured, and ultrastructures of ovary, pituitary and adrenal were observed by transmission electron microscopy. Results The serum concentrations of glucose, insulin, testosterone, triglyceride and cholesterol in androgen injection group and androgen injection+high-fat diet group were higher than those in control group. Polycystic changes of ovaries and increasing apoptosis in pituitaries and adrenals were observed in androgen injection group and androgen injection+high-fat diet group, and increasing lipids were found in androgen injection+high-fat diet group. Conclusion Androgen can induce the polycystic ovaries and increase the blood glucose in female rats. Androgen combined with high-fat diet may well meet the modeling requirements for polycystic ovary syndrome in female rats.

Key words: polycystic ovary syndrome, ultrastructure, androgen, high-fat diet