上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

敌百虫亚急性染毒对雄性小鼠的生殖毒性作用

唐 龙1，户 宜1，刘晓亮2，林子义1，张 妍1，施 蓉1，高 宇1   

  1. 上海交通大学 1.公共卫生学院， 上海 200025； 2.医学院附属上海儿童医学中心， 上海 200127
  • 出版日期:2013-12-28 发布日期:2014-01-02
  • 通讯作者: 高 宇, 电子信箱: gaoyu999@hotmail.com。
  • 作者简介:唐 龙（1989—）， 男， 本科生; 电子信箱: tanglong650516@163.com。
  • 基金资助:
    上海市卫生局基金（2008Y119）；上海市教委基金（06BZ004）；国家自然科学基金（30901171）；上海市自然科学基金（09ZR1416500）；上海市卫生局重点学科建设项目（12GWZX0401）；上海交通大学医学院校基金（09XJ21054，12XJ10006，12XJ10043）

Subacute effects of Trichlorfon on the reproductive function of male mice

TANG Long1, HU Yi1, LIU Xiao-liang2, LIN Zi-yi1, ZHANG Yan1, SHI Rong1, GAO Yu1   

  1. 1. School of Public Health, Shanghai Jiaotong University, Shanghai 200025, China; 2.Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
  • Online:2013-12-28 Published:2014-01-02
  • Supported by:
    Foundation of Shanghai Municipal Health Bureau, 2008Y119；Foundation of Shanghai Municipal Education Committee, 06BZ004； National Natural Science
    Foundation of China, 30901171； Natural Science Foundation of Shanghai, 09ZR1416500； Key Discipline Construction Project of Shanghai Municipal Health Bureau, 12GWZX0401； Foundation of Shanghai Jiaotong University School of Medicine, 09XJ21054, 12XJ10006, and 12XJ10043

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摘要：

目的 探讨有机磷农药敌百虫对雄性小鼠的生殖毒性作用。方法 选用雄性ICR小鼠40只，随机分为低剂量染毒组（2 mg/kg敌百虫）、中剂量染毒组（10 mg/kg敌百虫）、高剂量染毒组（50 mg/kg敌百虫）和对照组（等容积生理盐水），每组10只进行灌胃染毒，每天1次，连续30 d后脱椎处死小鼠，比较各组小鼠的精子活动度、精子畸形率以及睾丸组织的病理学改变，并分析染毒剂量与精子活动度和畸形率的关系。结果 与对照组比较，各染毒组小鼠的精子活动度均下降，其中精子活动度Ⅲ、Ⅳ级所占比例明显上升，且高剂量染毒组精子活动度为Ⅰ、Ⅱ级所占比例明显下降，差异均有统计学意义（P<0.05或P<0.01）。低剂量、中剂量和高剂量染毒组的精子畸形率分别为1.00%、2.06%和3.39%，均显著高于对照组的0.38%，差异有统计学意义（P<0.05）。精子活动度和畸形率均与染毒剂量显著相关（P<0.05，P<0.05）。组织病理学观察发现，中剂量和高剂量染毒组小鼠的睾丸曲细精管中各级精母细胞数量减少。结论 敌百虫暴露可对雄性小鼠产生一定的生殖毒性作用。

关键词: 敌百虫, 小鼠, 生殖毒性, 精子

Abstract:

Objective To explore reproductive toxicity of Trichlorfon in male mice. Methods Forty male ICR mice were randomly divided into three exposure groups (2, 10 and 50 mg/kg dose of Trichlorfon) and control group (equal volume of saline), and each group included 10 mice. All mice were exposed orally once per day for 30 d continuously, then the mice were sacrificed by cervical dislocation. The rates of sperm motility and sperm shape abnormality were measured, and histopathological changes of testis were observed. The relationship between dose of Trichlorfon and sperm motility and abnormality was analyzed. Results Compared to control group, the percentage of sperm motility in exposure groups changed remarkably, where the percentage of Ⅲ and Ⅳ grades increased in all exposure groups and Ⅰ and Ⅱ grades decreased in 50 mg/kg group (P<0.05, P<0.01). The percentage of sperm abnormality increased significantly (P<0.05) (control group: 0.38%, 2 mg/kg group: 1.00%, 10 mg/kg group: 2.06%, and 50 mg/kg group: 3.39%). The changes for sperm motility and abnormality were presented in a dose-dependent manner. Pathologic observation revealed that the number of spermatocytes in somniferous tubules was reduced in the 10 mg/kg and 50 mg/kg groups. Conclusion Exposure to Trichlorfon may cause reproductive toxicity in male mice.

Key words: Trichlorfon, mice, reproductive toxicity, sperm