上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2013, Vol. 33 ›› Issue (6): 796-.doi: 10.3969/j.issn.1674-8115.2013.06.020

• 论著（临床研究） • 上一篇    下一篇

汉族人群慢性牙周炎患者IL-13和IL-4基因多态性及连锁不平衡研究

陈 栋1,2，汪黎明2，张 洁2，束 蓉1   

  1. 1.上海交通大学 医学院附属第九人民医院牙周病科， 上海交通大学口腔医学院  上海市口腔医学重点实验室， 上海 200011； 2.上海市口腔病防治院牙周病科， 上海 200001
  • 出版日期:2013-06-28 发布日期:2013-06-28
  • 作者简介:陈栋(1969—)， 男， 硕士生， 主任医师; 电子信箱: chendong19690124@sohu.com。
  • 基金资助:

    上海市自然科学基金（10ZR1425900）；上海市卫生局基金（2009202）

Gene polymorphism and linkage disequilibrium of IL-13 and IL-4 in Chinese Han population with chronic periodontitis

CHEN Dong1,2, WANG Li-ming2, ZHANG Jie2, SHU Rong1   

  1. 1.Department of Periodontology, the Ninth People's Hospital, College of Stomatology, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China; 2.Department of Periodontology, Shanghai Stomatological Disease Center, Shanghai 200001, China
  • Online:2013-06-28 Published:2013-06-28
  • Supported by:

    Natural Science Foundation of Shanghai, 10ZR1425900; Shanghai Municipal Health Bureau Foundation, 2009202

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摘要：

目的 研究汉族人群慢性牙周炎患者白介素13（IL-13）和IL-4基因多态性分布情况及其与慢性牙周炎发病的可能关系。方法 采用基质辅助激光解吸离子化-飞行时间质谱法（MALDI-TOF-MS）对慢性牙周炎组（n=278）和健康对照组（n=324）IL-13基因中rs2070874和rs1800925及IL-4基因中rs2243248的单核苷酸多态性（SNP）进行检测，计算等位基因频率和基因型分布，并进行连锁不平衡及单倍型分析。结果 慢性牙周炎组与健康对照组rs1800925的TT基因型和T等位基因与rs2243248的GG基因型和G等位基因频率的差异有统计学意义（P<0.05）；两组间rs2070874 T/rs1800925 T/rs2243248 G单倍型及另外两种单倍型rs2070874 T/rs1800925 C/rs2243248 G与rs2070874 T/rs1800925 T/rs2243248 T的分布差异也有统计学意义（P<0.05）。结论 IL-13基因rs1800925和IL-4基因rs2243248的SNP可能与中国汉族人群慢性牙周炎的发病易感性相关。

关键词: 慢性牙周炎, 单核苷酸多态性, 单倍型, 白介素13, 白介素4

Abstract:

Objective To investigate the gene polymorphism of interleukin (IL)-13 and IL-4 in Chinese Han population with chronic periodontitis, and explore its potential relationship with chronic periodontitis. Methods Matrix-assisted laser desorption ionization time of flight mass spectrometer (MALDI-TOF-MS) was employed to detect the single nucleotide polymorphism (SNP) of rs2070874 and rs1800925 in IL-13 and rs2243248 in IL-14 in chronic periodontitis group (n=278) and healthy control group (n=324), the allele frequency and genotype distribution were calculated, and linkage disequilibrium and haplotype analysis was conducted. Results There were significant differences in the allelic frequency of rs1800925 TT genotype and T allele, as well as that of rs2243248 GG genotype and G allele between chronic periodontitis group and healthy control group (P<0.05). There were also significant differences in the distribution of rs2070874 T/rs1800925 T/rs2243248 G, rs2070874 T/rs1800925 C/rs2243248 G and rs2070874 T/rs1800925 T/rs2243248 T between two groups (P<0.05). Conclusion SNP of rs1800925 in IL-13 gene and rs2243248 in IL-4 gene may be correlated with the susceptibility to chronic periodontitis in Chinese Han population.

Key words: chronic periodontitis, single nucleotide polymorphism, haplotype, interleukin-13, interleukin-4