上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

T细胞淋巴瘤长链非编码RNA ST20-AS1的表达及其生物学作用

孙漪峰， 许彭鹏， 赵维莅   

  1. 上海交通大学 医学院附属瑞金医院血液科， 上海血液学研究所， 上海 200025
  • 出版日期:2016-09-28 发布日期:2016-10-31
  • 通讯作者: 赵维莅, 电子信箱: zhao.weili@yahoo.com。
  • 作者简介:孙漪峰（1989—）， 男， 硕士生; 电子信箱: sunyifeng89@gmail.com。
  • 基金资助:

    国家自然科学基金（81325003, 81520108003, 81201863）；上海市科学技术委员会（14430723400, 14140903100）；瑞金医院优秀青年教师（800000000003）；上海交通大学晨星青年学者计划；上海市青年医师培养资助计划；上海市教育委员会高峰高原学科建设计划（20152208）

Expression and biological effect of long non-coding RNA  ST20-AS1  in T-cell lymphoma

SUN Yi-feng, XU Peng-peng, ZHAO Wei-li   

  1. Shanghai Institute of Hematology, Department of Hematology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine， Shanghai  200025, China
  • Online:2016-09-28 Published:2016-10-31
  • Supported by:

    National Natural Science Foundation of China, 81325003, 81520108003, 81201863; Shanghai Commission of Science and Technology, 14430723400, 14140903100； Ruijin Hospital Outstanding Young Teachers, 800000000003； Shanghai Jiao Tong University Morningstar Award； Shanghai Young Doctor Training Program； Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support， 20152208

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摘要：

目的·检测T细胞淋巴瘤患者长链非编码RNA（lncRNA）ST20-AS1的表达，并探索其对T细胞淋巴瘤细胞增殖和侵袭性的影响。方法·运用lncRNA芯片筛选出ST20-AS1作为研究的lncRNA，通过芯片数据发现ST20-AS1的高表达与NOTCH信号通路的关系；Real-time RT-PCR方法验证ST20-AS1在T细胞淋巴瘤患者中的表达水平，并验证其对NOTCH通路的激活作用；构建ST20-AS1稳定表达的T淋巴瘤Jurkat细胞株，进行细胞增殖和Transwell侵袭实验，观察细胞增殖和侵袭性的改变。结果·ST20-AS1在T淋巴瘤患者中呈高表达，ST20-AS1高表达患者的NOTCH通路被显著激活。在Jurkat细胞株中发现，ST20-AS1高表达活化NOTCH信号通路，增强细胞增殖和侵袭能力。利用siRNA敲低NOTCH1的表达后，ST20-AS1高表达细胞株的增殖和侵袭能力降低。结论·ST20-AS1激活NOTCH信号通路，促进淋巴瘤细胞的增殖和侵袭性。

关键词: T细胞淋巴瘤, ST20-AS1, NOTCH信号通路, 细胞增殖, 细胞侵袭

Abstract:

Objective·To measure the expression of ST20-AS1, a long non-coding RNA (lncRNA) in patients with T-cell lymphoma, and to explore its effects on the proliferation and invasion of T-cell lymphoma cells. Methods·Study lncRNA was screened using the lncRNA array. The association between high expression of ST20-AS1 and NOTCH signaling pathway was detected via the data from the lncRNA array. The expression level of ST20-AS1 in T-cell lymphoma patients was assessed by real-time RT-PCR and its activation effect on NOTCH signaling pathway was verified. ST20-AS1-overexpressing T-cell lymphoma cell line Jurkat was constructed and cell proliferation test and Transwell assay were performed to observe changes in cell proliferation and invasion. Results·ST20-AS1 was overexpressed and NOTCH signaling pathway was significantly activated in T-cell lymphoma patients. The overexpression of ST20-AS1 activated the NOTCH signaling pathway and promoted lymphoma cell proliferation and invasion in Jurkat. When NOTCH1 was knocked down by siRNA, cell proliferation and invasion was reduced in ST20-AS1-overexpressing cell line Jurkat. Conclusion·ST20-AS1 can activate the NOTCH signaling pathway and promote lymphoma cell proliferation and invasion.

Key words: T-cell lymphoma, ST20-AS1, NOTCH signaling pathway, cell proliferation, cell invasion