上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (3): 305-.

• 论著（基础研究） • 上一篇    下一篇

种植窗期人子宫内膜组织SDF-1α/CXCR4的表达

马 珏1, 李 路1, 高晓红1, 吴 煜1, 陆 湘1, 陈秋菊2, 孙晓溪1   

  1. 1. 上海交通大学 医学院国际和平妇幼保健院生殖医学中心, 上海 200030；2. 上海市计划生育科学研究所, 上海 200032
  • 出版日期:2010-03-25 发布日期:2010-03-24
  • 通讯作者: 孙晓溪, 电子信箱: sun_xx@hotmail.com。
  • 作者简介:马 珏（1980—）, 女, 住院医师, 硕士；电子信箱: mary_0802@yahoo.com.cn。
  • 基金资助:

    上海市教委创新项目（06BZ051）

Expression of SDF-1α/CXCR4 in human endometria of peri-implantation phase

MA Jue1, LI Lu1, GAO Xiao-hong1, WU Yu1, LU Xiang1, CHEN Qiu-ju2, SUN Xiao-xi1   

  1. 1. Department of Reproductive Medicine, International Peace Maternity &|Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China；2. Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
  • Online:2010-03-25 Published:2010-03-24
  • Supported by:

    Shanghai Education Committee Foundation, 06BZ051

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摘要：

目的 研究不同卵巢反应性的种植窗期子宫内膜组织中基质细胞衍生因子-1α（SDF-1α）及其受体CXCR4的表达。方法 收集种植窗期子宫内膜组织（n=17），根据卵巢反应性分为卵巢高反应组（n=6）、卵巢中等反应组（n=6）和正常对照组（n=5）。应用RT-PCR、Real-time PCR、免疫组织化学染色和Western blotting方法，检测并比较各组种植窗期子宫内膜组织SDF-1α/CXCR4 mRNA和蛋白表达。结果 免疫组织化学染色显示，CXCR4在种植窗期子宫内膜组织中高表达；SDF-1α在内膜腺体和腔上皮细胞中表达，而在间质细胞中不表达。各组子宫内膜组织SDF-1α/CXCR4 mRNA和蛋白表达比较，差异均无统计学意义(P>0.05)。结论 高甾体激素对种植窗期子宫内膜趋化因子SDF-1α及其受体CXCR4的表达无明显影响。提示SDF-1α/CXCR4可能不参与甾体激素对种植窗期CD56brightCD16-NK细胞募集的调控。

关键词: 子宫内膜, 种植窗期, 趋化因子, 基质细胞衍生因子-1α, CXCR4, 甾体激素

Abstract:

Objective To investigate the expression of stromal cell-derived factor-1α (SDF-1α) and CXCR4 under different ovarian responses in human endometria of peri-implantation phase. Methods Endometria of peri-implantation phase (n=17) were collected, and were divided into moderate ovarian response group (n=6), high ovarian response group (n=6) and normal control group (n=5). The expression of SDF-1α/CXCR4 mRNA and protein in endometria of peri-implantation phase was detected and analysed by RT-PCR, Real-time PCR, immunohistochemistry and Western blotting. Results It was revealed by immunohistochemistry that CXCR4 was highly expressed in endometrial tissues, whereas SDF-1α was confined to glandular and celomic epithelial cells instead of stromal cells. There was no significant difference in the expression of SDF-1α/CXCR4 mRNA and protein in human endometria among each group (P>0.05). Conclusion High levels of steroid hormone do not affect expression of SDF-1α/CXCR4 in human endometria of peri-implantation phase, indicating that SDF-1α/CXCR4 may not participate in the recruitment of CD56brightCD16-NK cells to endometria by steroid hormone.

Key words: endometrium, peri-implantation phase, chemokine, stromal cell-derived factor-1α, CXCR4, steroid hormone