上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (4): 419-.

• 论著（基础研究） • 上一篇    下一篇

寻常型天疱疮皮损组织中Th17细胞相关细胞因子的表达

苏文婷1, 王宏林2, 费 烨1, 汪蓓青1, 徐 慧1, 陈向东1   

  1. 上海交通大学 医学院 1. 第九人民医院皮肤科, 上海 200011, 2. 上海市免疫学研究所, 上海 200025
  • 出版日期:2010-04-25 发布日期:2010-04-26
  • 作者简介:苏文婷（1986—）, 女, 硕士生；电子信箱: suwenting1986@yahoo.com.cn。

Expression of Th17 cell associated cytokines in pemphigus vulgaris lesions

SU Wen-ting1, WANG Hong-lin2, |FEI Ye1, WANG Bei-qing1, XU Hui1, CHEN Xiang-dong1   

  1. 1. Department of Dermatology, The Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, China；2. Shanghai Institute of Immunology, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-04-25 Published:2010-04-26

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摘要：

目的 研究Th17细胞相关细胞因子IL-23和IL-17在寻常型天疱疮（PV）发病机制中的作用。方法 采用免疫荧光染色法分析PV患者皮损组织和健康人皮肤组织中Th17细胞相关因子IL-17和IL-23的表达及CD163+巨噬细胞的分布。结果 IL-23+细胞的染色与CD163+巨噬细胞的染色重叠。PV患者真皮中IL-23+CD163+细胞和IL-17+细胞在每个视野（×200）中的平均数分别为80.17±5.42和48.17±8.03，而正常人真皮中分别为29.50±4.45和9.50±3.35，两组差异均有统计学意义（P<0.01）。真皮层中IL-23+细胞数和IL-17+细胞数存在相关性（r=0.945，P<0.001）。结论 CD163+巨噬细胞是IL-23重要分泌源；IL-23和IL-17在PV患者皮损组织中高表达，IL-23/IL-17细胞因子轴在PV发病机制中可能起着非常重要的作用。

关键词: 寻常型天疱疮, 巨噬细胞, Th17细胞, IL-23, IL-17

Abstract:

Objective To investigate the role of Th17 cell associated cytokines of IL-23 and IL-17 in the pathogenesis of pemphigus vulgaris. Methods Immunofluorescence staining was performed to analyse the distribution of CD163+ macrophages and expression of IL-23 and IL-17 in pemphigus vulgaris lesions and healthy human skin tissues. Results The staining of IL-23+cells is overlapped with that of CD163+ macrophages. The mean numbers of IL23+CD163+ cells and IL-17+ cells were 80.17±5.42 and 48.17±8.03 in each field of view (×200) in dermis of pemphigus vulgaris, while those in healthy human dermis were 29.50±4.45 and 9.50±3.35, with significant differences between dermis of pemphigus vulgaris and healthy human dermis (P<0.01). The number of IL23+ cells was related to that of IL-17+ cells in dermis (r=0.945, P<0.001). Conclusion IL-23 is mainly secreted by CD163+ macrophages. IL-23 and IL-17 are overexpressed in pemphigus vulgaris lesions. IL-23/IL-17 cytokine axis may play an important role in the pathogenesis of pemphigus vulgaris.

Key words: pemphigus vulgaris, macrophages, Th17 cell, IL-23, IL-17