上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (3): 275-.doi: 10.3969/j.issn.1674-8115.2011.03.006

• 论著（基础研究） • 上一篇    下一篇

雷帕霉素对人非小细胞肺癌细胞株A549生长及增殖的影响

李文艳, 朱 珺   

  1. 上海交通大学附属胸科医院药剂科, 上海 200030
  • 出版日期:2011-03-28 发布日期:2011-03-29
  • 作者简介:李文艳（1978—）, 女, 药师, 硕士；电子信箱: lwy2975@126.com。

Effects of rapamycin on growth and proliferation of human non-small cell lung cancer cell line A549

LI Wen-yan, ZHU Jun   

  1. Department of Pharmacy, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China
  • Online:2011-03-28 Published:2011-03-29

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摘要：

目的 探讨雷帕霉素对人非小细胞肺癌细胞株A549生长和增殖的影响。方法 分别以0（阴性对照组）、0.01、0.02、0.05、0.1、0.2、0.5、1、5 μmol/L雷帕霉素干预A549细胞（雷帕霉素干预组）24、48、72 h，MTT比色法检测细胞活力。分别以0（阴性对照组）、0.01、0.05、0.1、0.5、1  μmol/L雷帕霉素干预A549细胞（雷帕霉素干预组）48 h，光学显微镜观察细胞形态变化，PI单染流式细胞仪分析细胞周期。结果 MTT比色法检测结果显示：雷帕霉素干预组细胞生长抑制率显著高于阴性对照组（P<0.05），其中以5 μmol/L雷帕霉素干预72 h 组的细胞生长抑制率最高。与阴性对照组比较，经不同浓度雷帕霉素干预48 h后的A549细胞变圆缩小，伪足缩短，细胞间距增大；细胞周期检测结果显示：与阴性对照组比较，雷帕霉素干预组G0/G1期细胞百分率明显增加，S期细胞百分率显著减少(P<0.05)。结论 雷帕霉素对人肺癌细胞株A549的生长和增殖具有明显抑制作用，并呈现一定的时间和浓度依赖性。

关键词: 雷帕霉素, 肺癌, 增殖, 细胞周期

Abstract:

Objective To investigate the effects of rapamycin on the growth and proliferation of human non-small cell lung cancer cell line A549. Methods A549 cells were cultured with 0 (negative control group), 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 and 5 μmol/L rapamycin (rapamycin intervention group) for 24 h, 48 h and 72 h, and cell viability was detected by MTT assay. A549 cells were cultured with 0 (negative control group), 0.01, 0.05, 0.1, 0.5 and 1 μmol/L rapamycin (rapamycin intervention group) for 48 h, cell morphology was observed by light microscopy, and cell cycle was analysed by flow cytometry with PI simple staining. Results MTT assay revealed that the cell growth inhibition rate in rapamycin intervention group was significantly higher than that in negative control group (P<0.05), and the cell growth inhibition rate was most significant after culture with 5 μmol/L rapamycin for 72 h. Compared with negative control group, A549 cells tended to maintain a round shape by contracting the pseudopod, and the gaps between cells enlarged after culture with different concentrations of rapamycin for 48 h. Cell cycle detection indicated that compared with negative control group, the percent of cells in G0/G1 phase significantly increased, and the percent of cells in S phase significantly decreased in rapamycin intervention group (P<0.05). Conclusion Rapamycin has significant inhibitory effects on the growth and proliferation of human lung cancer cell line A549 in a time- and concentration-dependent manner.

Key words: rapamycin, lung cancer, proliferation, cells cycle