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       2019, 39 (6): 1-.  
    Abstract1103)      PDF(pc) (2415KB)(225)       Save
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    Analysis of clinicopathological factors of spread through air spaces in stage Ⅰ A lung adenocarcinoma
    CHEN Liang, WANG Zhe-xin, YAO Feng
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (07): 957-961.   DOI: 10.3969/j.issn.1674-8115.2020.07.015
    Abstract857)      PDF(pc) (6047KB)(161)       Save
    Objective · To investigate the clinicopathological risk factors of spread through air space (STAS) in stage Ⅰ A lung adenocarcinoma. Methods · The clinical data of patients with stage Ⅰ A lung adenocarcinoma who underwent lung surgery in Shanghai Chest Hospital, Shanghai Jiao Tong University from Jan. 2018 to Oct. 2019 were retrospectively collected. According to whether STAS happened, the patients were divided into STAS group and non-STAS group. Univariate analysis and binary Logistic regression analysis were used to analyze the risk factors of STAS in stage Ⅰ A lung adenocarcinoma between the two groups. Results · A total of 1 365 patients with stage Ⅰ A lung adenocarcinoma were included, including 1 312 patients without STAS and 53 patients with STAS. Univariate analysis showed that there were significant differences in gender and major subtypes of lung adenocarcinoma between the two groups (both P=0.000), but there was no significant difference in T stage between the two groups. Binary Logistic regression analysis showed that male and non-lepidic subtypes were independent risk factors. Conclusion · For the treatment of stage Ⅰ A lung adenocarcinoma, especially those with micropapillary subtype, the clinicians should pay attention to the possibility of STAS.
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    Palmitoylation of PD-L1 is a new target for cancer immunotherapy
    The research group of XU Jie
       2019, 39 (7): 689-.   DOI: 10.3969/j.issn.1674-8115.2019.07.001
    Abstract806)      PDF(pc) (3490KB)(402)       Save
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    Body composition analysis in pregnant women and its clinical applications
    OU Hong-hui, LI Hua-ping
       2019, 39 (11): 1341-.   DOI: 10.3969/j.issn.1674-8115.2019.11.021
    Abstract783)      PDF(pc) (6145KB)(270)       Save
    The maternal components are constantly changing with gestational weight gains during pregnancy. Body composition analysis is a method for evaluating the maternal body composition accurately during pregnancy, models of which consist of fat mass, extracellular water, intracellular water, inorganic salts and protein basically. Among various methods to body composition analysis, bioelectrical impedance analysis is an important method for pregnant women. Many studies have reported about clinical applications based on correlations between maternal individual components and gestational diseases. This article reviews the researches on clinical applications of body composition analysis to pregnant women.
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    Advanced research of kynurenine pathway mechanism in suicide of major depressive disorder
    ZHAO Dong-mei1, 2*, DING Lei1*, LIU Hong-ye1, 3, PENG Dai-hui1
       2019, 39 (7): 805-.   DOI: 10.3969/j.issn.1674-8115.2019.07.021
    Abstract762)      PDF(pc) (6501KB)(236)       Save
    Major depressive disorder (MDD) is one of the world′s major chronic and disabling mental diseases.2030, MDD is expected to be the top of all the disease burden in the world, with high prevalence, high recurrence rate, high disability rate, and high suicide rate. Suicide is the most serious consequence of MDD. Current studies showed that inflammatory levels in the central nervous system and peripheral blood of patients with MDD were higher, and increased more significantly in depressive patients with suicidal ideation or behavior. Related researches showed that increased levels of inflammatory cytokines were associated with dysregulation of kynurenine metabolic pathway, leading to imbalances in neurometabolites, such as an excess of the neurotoxic product quinolinic acid and a decrease in the protective neuropeptide picolinic acid. This paper reviews kynurenine metabolic pathway, expecting to identify the biomarkers of MDD patients with suicide.
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    Application of repetitive transcranial magnetic stimulation in the treatment of obsessive-compulsive disorder
    LI Pu-yu, WANG Zhen
       2019, 39 (12): 1477-.   DOI: 10.3969/j.issn.1674-8115.2019.12.023
    Abstract716)      PDF(pc) (10090KB)(211)       Save
    At present, a considerable proportion of patients with obsessive-compulsive disorder (OCD) cannot be effectively relievedstandard drug and psychotherapy, so researchers have turned their attention to new directions of physiotherapy. Repetitive transcranial magnetic stimulation (rTMS), as an adjuvant therapy for refractory OCD, is a non-invasive nerve stimulation technique. Many studies have shown that rTMS is effective in the treatment of OCD. However, there were also disputes in the selection of stimulation targets, parameter settings and so on. This article systematically combs the setting and application of standard rTMS in the treatment of OCD, and comprehensive therapeutic effect of rTMS, and then discusses the deficiency of treatment so far, into put forward the future development direction and promote clinical treatment progress.
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    Predicting the trend of the COVID-19 outbreak and timely grading the current risk level of epidemic based on moving average prediction limits
    HE Hao, HE Yun-ting, ZHAI Jing, WANG Xiao-jin, WANG Bing-shun
       2020, 40 (4): 422-.   DOI: 10.3969/j.issn.1674-8115.2020.04.002
    Abstract709)      PDF(pc) (10240KB)(259)       Save
    Objective · To establish a practical data-driven method that helps predict the evolutionary trend of the coronavirus disease 2019 (COVID-19) epidemic, track and prejudge the current risk classification of the epidemic area, and provide a quantitative evidence for precision prevention and control strategies. Methods · A moving average prediction limit (MAPL) method was established based on the moving average method. The previous severe acute respiratory syndrome (SARS) epidemic data was used to verify the practicability of the MAPL method for predicting epidemic trends and quantitative risk.tracking the COVID-19 outbreak epidemic data publicly reported since January 16, 2020, the MAPL method was used for timely epidemic trend prediction and the risk classification. Results · According to the MAPL analysis, the national epidemic of COVID-19 peaked in early February 2020. After active prevention and control in early stages, the overall epidemic situation in the country showed a downward trend mid-February to mid-March. Compared with Hubei Province, the number of new cases in non-Hubei region declined rapidly in mid-February, but then increased slightly. The analysis of imported cases since March showed that there was a medium to high level of epidemic import risk in the near future. It is recommended to take corresponding prevention and control measures to prevent the epidemic spreading again. Conclusion · The MAPL method can assist in judging the epidemic trend of emerging infectious diseases and predicting the risk levels in a timely manner. Each epidemic district may implement a differentiated precision prevention and control strategies according to the local classification of epidemic risk. Since March, attention should be paid to the prevention and control of imported risks.
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    Structure and degradation of circular RNAs regulate PKR activation in innate immunity
    The research group of SHEN Nan
       2019, 39 (8): 809-.   DOI: 10.3969/j.issn.1674-8115.2019.08.001
    Abstract687)      PDF(pc) (3832KB)(205)       Save
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    Aerosol transmission prevention of novel coronavirus in dental clinics
    RAN Shu-jun, LIANG Jing-ping
       2020, 40 (3): 282-.   DOI: 10.3969/j.issn.1674-8115.2020.03.002
    Abstract686)      PDF(pc) (7143KB)(239)       Save
    Corona virus disease 2019 (COVID-19), which broke out in Wuhan, HuBei in December, 2019, spread around the country in a short term. Some infected patients suffered severe acute respiratory syndrome. It has become a major public health event of international concern. COVID-19 patients are the main source of transmission and most people get infection easily, so the current main prevention method is interrupting the pathway of transmission. The dental treatments are very special becathese operations produce substantial aerosols and lead a high risk of cross-infection, which brings challenge for epidemic prevention and control. In this paper, the characteristics of aerosols and prevention methods of aerosol transmission in dental clinics are mainly discussed.
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    Two-photon in vivo imaging of blood brain barrier injury in the ultra-early stage of cerebral ischemic stroke
    HUANG Ting-ting, LI Yan, ZHANG Yue-man, ZHOU Na-ying, FAN Ren-hui, LI Pei-ying
       2019, 39 (9): 998-.   DOI: 10.3969/j.issn.1674-8115.2019.09.010
    Abstract677)      PDF(pc) (8735KB)(304)       Save
    Objective · To evaluate the effect of two-photon in vivo imaging on detecting the blood brain barrier (BBB) injury in the ultra-early stage of cerebral ischemic stroke. Methods · Twelve clean grade C57BL/6 healthy male mice aged 8-12 weeks were randomly divided into Sham group and middle cerebral artery occlusion (MCAO) group, which were sham operated or middle cerebral artery occluded, respectively. After 60 min of ischemia, MCAO mice were treated with reperfusion for 30-60 min after the suture being removed. The vessels and the neutrophils of mice in the two groups were labeled intravenously with Alexa-Fluora-488 conjugated dextran and rhodamine 6G, respectively. The integrity of BBB was detectedintravenous injection of tetramethylrhodamine-5-maleimide (TMR). Before and after the stroke, the real-time changes of the fluorescence intensity of the inside and outside cerebral vessels of mice in the MCAO group were observedtwo-photon fluorescence microscopy. Results · The fluorescence intensity of TMR in the external cerebrovascular of mice in the MACO group was significantly increased within 30-60 min after stroke (P0.000), suggesting there existed tracer leakage. Compared with the Sham group, the movement of neutrophils in the blood vessels of mice in the MACO group was significantly slowed down (P0.000). Conclusion · Two-photon in vivo imaging can be used to detect the BBB injury in the ultra-early stage of cerebral ischemic stroke, which provides a certain reference value for clinical application.
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    Mitigate the effects of home confinement on children during the COVID-19 outbreak
    WANG Guang-hai, ZHANG Yun-ting, ZHAO Jin, ZHANG Jun, JIANG Fan
       2020, 40 (3): 279-.   DOI: 10.3969/j.issn.1674-8115.2020.03.001
    Abstract643)      PDF(pc) (3992KB)(284)       Save
    More than 220 million Chinese children and adolescents are confined to their homes becaof the corona virus disease 2019 (COVID-19) outbreak. Thanks to the strong administrative system in China, the emergency home schooling plan has been rigorously implemented. Although these measures and efforts are highly commendable and necessary, there are reasons to be concerned becaprolonged school closure and home confinement during a disease outbreak might have negative effects on childrens physical and mental health. It is the responsibility and keen interests of all stakeholders, governments to parents, to ensure that the physical and mental impacts of the COVID-19 epidemic on children and adolescents are kept minimal.
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    Physiological function of cholesterol sulfate and its role in related diseases
    Yue-ting JIANG, Jia-ying NI, Shen-rui GUO, Han LI, Yu-jia ZHUANG, Feng WANG
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2021, 41 (3): 371-375.   DOI: 10.3969/j.issn.1674-8115.2021.03.015
    Abstract639)   HTML10)    PDF(pc) (1069KB)(191)       Save

    Cholesterol sulfate (CS), synthesized by sulfotransferase (SULT) 2B1b, is an important steroid sulfate and plays important physiological roles in the human body. It is widely distributed in human body, such as skin, adrenal gland, liver, lung, brain and endometrium. CS participates in the formation of cornified envelopes and the expression of keratinocyte differentiation markers in the epidermis, thereby regulating epidermal desquamation and barrier function. CS inhibits T cell signaling during thymocyte development in the immune system, and CS/ Cholesterol ratio directly affects thymic selection for T cells, thereby participating in the shaping of T cell receptor repertoire. CS regulates brain metabolism and exerts neuroprotective effects by reducing oxidative stress, maintaining mitochondrial membrane stability and increasing energy reserves. In addition, CS also contributes to the development of many diseases by regulating the activity of functional proteins. The deletion and mutation of steroid sulfatase (STS) gene, which catalyzes the desulfurization of CS, directly leads to the occurrence of X-linked ichthyosis. CS is involved in the development of Alzheimer's disease by promoting the aggregation of amyloid β - protein (Aβ). CS regulates gluconeogenesis by inhibiting the activation of hepatocyte nuclear factor 4α (HNF4α). Thus CS is expected to treat type 2 diabetes. CS has abnormal expression in a variety of cancers, and can interact with matrix metalloproteinase-7 (MMP-7) to induce the aggregation and metastasis of cancer cells. The main challenges and research priorities at this stage are to reveal specific molecular mechanisms under different physiological and pathological conditions and to design feasible clinical treatments.

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    A source-seeking analysis and its implication on the transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
    WANG Xiao-yu, CUI Li
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)   
    Online available: 27 February 2020

    Identification of hub genes and key pathways in breast cancersurvival-based bioinformatics analysis
    CHEN Si, LIU Chun-liang, ZHAO Qian, SUN Hai-peng, LIU Yun-xia
       2020, 40 (3): 294-.   DOI: 10.3969/j.issn.1674-8115.2020.03.004
    Abstract632)      PDF(pc) (10479KB)(353)       Save
    Objective · To identify hub genes and key pathways in breast cancerbioinformatics analysis that integrated gene data with clinical survival analysis. Methods · Three gene profilings downloaded Gene Expression Omnibus (GEO) were used to identify differentially expressed genes (DEGs) in breast cancer. Kaplan-Meier plotter was used to identify the DEGs that were significantly associated with overall survival in breast cancer. Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed. Next, hub genes were identified the protein-protein interaction (PPI) network. Oncomine and the Human Protein Atlas (HPA) database were used to validate the of the hub genes. The s of hub genes in MDA-MB-231 cells and MCF-10A cells were detectedquantitative real-time PCR (qPCR). Results · Among the DEGs, 262 genes were significantly correlated with overall survival of breast cancer patients. The results of GO functional analysis and KEGG pathway analysis showed that these genes were associated with nuclear division, cell division and chromosome segregation, and were mainly enriched on the pathways such as cell cycle, FoxO signaling pathway and oocyte meiosis. PPI network construction identified ten hub genes. They were all highly expressed in breast cancer, which were validatedthe databases. The results of qPCR showed that 8 out of 10 hub genes were highly expressed in breast cancer cells. Conclusion · The hub genes and key pathways involved in the development of breast cancer are identifiedsurvival-based bioinformatics analysis, which are mainly associated with cell cycle regulation and cell division.
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    SIRT1 signaling pathway in bone metabolism
    YANG Yi-qi, TANG Ting-ting
       2019, 39 (11): 1335-.   DOI: 10.3969/j.issn.1674-8115.2019.11.020
    Abstract630)      PDF(pc) (9226KB)(333)       Save
    Osteoporosis is a bone disease characterizedlow bone mass and deteriorated bone microstructure, which could be related to the disorders of energy metabolism and bone senescence. Silent mating-type information regulator 2 homolog 1 (SIRT1) is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase that regulates cell senescence, energy metabolism and bone remodeling. SIRT1 could be activated not onlyadenosine 5-monophosphate (AMP)-activated protein kinase (AMPK), c-Jun N-terminal kinase 1 (JNK1) and casein kinase 2 (CK2), but alsosmall-molecular drugs such as resveratrol. All these kinases and drugs can affect bone metabolism. Recent findings indicate that SIRT1 signaling pathway plays a direct role in bone metabolism, but the underlying mechanism remains unclear. This paper reviews the structure and function of SIRT1, and the role of SIRT1 in bone metabolism, and discusses the potential of SIRT1 signaling pathway as a new therapeutic target in osteoporosis.
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    Research progress on the promoting effect of interleukin-11 on tumors
    PAN De-shen, LI Deng, SHAO Yi
       2020, 40 (4): 548-.   DOI: 10.3969/j.issn.1674-8115.2020.04.023
    Abstract604)      PDF(pc) (8498KB)(195)       Save
    According to the lastest statistics, the overall morbidity and mortality of cancer in China still show an upward trend compared with historical data. An in-depth understanding of the molecular mechanisms of tumorigenesis and development is important to formulate future treatment strategies. Interleukin 11 (IL-11) is a member of cytokines that traditionally promote megakaryocyte maturation and regulate immune activity. In recent years, the promoting effect of IL-11 on tumor has been gradually discovered. This review mainly expounds that IL-11 is regulatedtransforming growth factor-β/drosophila mothers against decapentaplegic protein (TGF-β/Smad) pathway, and may play a role in tumorigenesis, drug resistance, metastasis and tumor microenvironment through signal transduction pathways such as Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway and phosphatidylinositol 3 kinase/protein kinase B (PI3K/AKT) pathway, and explores the application prospect of interfering IL-11 signal transduction in tumor therapy.
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    Management on prevention of new coronavirus infection in periodontal treatment
    QIAN Jie-lei, SHU Rong
       2020, 40 (2): 145-.   DOI: 10.3969/j.issn.1674-8115.2020.02.001
    Abstract594)      PDF(pc) (6287KB)(325)       Save
    Currently, corona virus disease 2019 (COVID-19) causedsevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly contagious disease. The epidemic situation was so severe that the accumulative number of confirmed diagnoses in China reached more than seventy thousand24:00, Feb. 18, 2020. This paper, based on the etiological and epidemiological characteristics of the virus, introduces the infection prevention protocols for COVID-19 before, during and after the periodontal treatment for the reference of stomatologists. Proper prevention methods can reduce the risks of nosocomial infection, prevent further spreading, ensure the safety of the medical staff and patients, and then ensure both medical quality and safety.
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    Clinical progress of noninvasive follicular thyroid neoplasm with papillary-like nuclear features
    ZENG Su, XU Pei-pei, GUO Ming-gao
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (07): 968-973.   DOI: 10.3969/j.issn.1674-8115.2020.07.017
    Abstract582)      PDF(pc) (9333KB)(68)       Save
    In 2015, the United States and Canadian Academy of Pathology re-evaluated noninvasive encapsulated follicular variant of papillary thyroid carcinomas (NI-EFVPC), and then the new terminology of "noninvasive follicular thyroid neoplasm with papillary-like nuclear features" (NIFTP) was initially introduced to replace NI-EFVPTC. In 2017, the World Health Organization also used NIFTP in the endocrine tumor classification. NIFTP is an encapsulated or clearly delimited noninvasive neoplasm with a follicular growth pattern and nuclear features of papillary thyroid carcinoma. Ultrasonography, cytology examination and genetic test are helpful to identify NIFTP, but the results overlap with invasive encapsulated follicular variant of papillary thyroid carcinoma. This paper mainly reviews the latest research in the clinical features, diagnosis and treatment features and prognosis of NIFTP.
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    Construction of inducible CRISPR/Cas9 system for studying gene function in mouse
    Yan-na ZHAO, Rong QIU, Nan SHEN, Yuan-jia TANG
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2021, 41 (3): 297-301.   DOI: 10.3969/j.issn.1674-8115.2021.03.002
    Abstract581)   HTML22)    PDF(pc) (1811KB)(143)       Save
    Objective

    ·To construct inducible CRISPR/Cas9 system for studying gene function in mouse immune cells, combining Dox-inducible single guide RNA (sgRNA) expression vector with Cas9 transgenic mice.

    Methods

    ·U6-TetO-sgRNA and EF1α-T2A-Puro-BFP-T2A-TetR fragments were obtained by gene synthesis. The two synthetic fragments were assembled into the retroviral vector backbone by using homologous recombination. sgRNA targeting protein coding region of F4/80 and non-targeting control (NC) were designed. Bone marrow cells were isolated from Cas9 transgenic mice and transfected with retrovirus expressing sgRNA. The experimental conditions were divided into Dox-added group (Dox +) and non Dox-added group (Dox-). The knockout effect was tested by flow cytometry and T7 endonuclease Ⅰ (T7EⅠ) experiments.

    Results

    ·①Dox-inducible sgRNA retroviral vector and Cas9 transgenic mice were successfully constructed. ② The result of flow cytometry showed that F4/80 was only knocked out in the F4/80 Dox+ population, but not in NC Dox-, NC Dox+ and F4/80 Dox- populations. ③ T7EⅠ results showed that the DNA was cut into two bands in the F4/80 Dox+ group, while the DNA band was intact in the F4/80 Dox- group.

    Conclusion

    ·An inducible CRISPR/Cas9 system combining Dox-inducible sgRNA retroviral vector with Cas9 transgenic mice are successfully constructed. With this system, inducible gene knockout in mouse immune cells are successfully achieved.

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    Application of artificial intelligence in assisted diagnosis and treatment of cardiovascular disease
    LU Yan-qiao, SHEN Lan, HE Ben
       2020, 40 (2): 259-.   DOI: 10.3969/j.issn.1674-8115.2020.02.019
    Abstract579)      PDF(pc) (6215KB)(413)       Save
    With the continuous development of science and technology, artificial intelligence (AI) with self-directed learning, induction and deduction, low error rate and other advantages has become the most popular emerging technology. In the traditional medical field, especially in cardiovascular diseases with high incidence, AI can not only provide auxiliary diagnosis and treatment for patients, but also enable doctors to optimize traditional risk assessment models which can predict the evolution of the disease better. Thereby, AI can provide more convenience and new perspectives for clinical work. This paper reviews the research progress of AI in the cardiovascular field in recent years.
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